| JANICZEK, Oldřich, Blanka POKORNÁ, Jana ZEMANOVÁ a Martin MANDL. Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans. Journal of Biotechnology. Amsterdam: Elsevier, 2005, roč. 117, č. 1, s. 293-298. ISSN 0168-1656. |
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@article{570573,
author = {Janiczek, Oldřich and Pokorná, Blanka and Zemanová, Jana and Mandl, Martin},
article_location = {Amsterdam},
article_number = {1},
keywords = {Affinity chromatography; Acidithiobacillus; thiosulfate dehydrogenase; immobilized cytochrome c},
language = {eng},
issn = {0168-1656},
journal = {Journal of Biotechnology},
title = {Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans},
volume = {117},
year = {2005}
}
TY - JOUR
ID - 570573
AU - Janiczek, Oldřich - Pokorná, Blanka - Zemanová, Jana - Mandl, Martin
PY - 2005
TI - Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans
JF - Journal of Biotechnology
VL - 117
IS - 1
SP - 293-298
EP - 293-298
PB - Elsevier
SN - 01681656
KW - Affinity chromatography
KW - Acidithiobacillus
KW - thiosulfate dehydrogenase
KW - immobilized cytochrome c
N2 - Three matrixes were used for immobilizing the cytochrome c: Sepharose CL-4B, Silasorb SPH amine and a laboratory-prepared new matrix based on crosslinked triazine (2,4,6-tris (aminoethylamine)-1,3,5-triazine) (TAT). Cytochrome c was immobilized on the matrixes by several procedures and the amount of incorporated cytochrome c was determined. Cytochrome c immobilized on Sepharose CL-4B with periodate activation, cytochrome c immobilized on Silasorbamine with carbodiimide activation and cytochrome c immobilized on crosslinked triazine were suitable for purification of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans. The yield with all matrixes was about 90%. The purification factor of the above matrixes was about 15. A new matrix based on TAT with cytochrome c represented a suitable way for thiosulfate dehydrogenase purification.
ER -
JANICZEK, Oldřich, Blanka POKORNÁ, Jana ZEMANOVÁ a Martin MANDL. Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans. \textit{Journal of Biotechnology}. Amsterdam: Elsevier, 2005, roč.~117, č.~1, s.~293-298. ISSN~0168-1656.
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