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@article{570714,
author = {Lochman, Jan and Kašparovský, Tomáš and Damborský, Jiří and Osman, Hanan and Ponchet, MIchel and Mikeš, Vladimír and Chaloupková, Radka},
article_number = {17},
keywords = {Elicitor; Phytophthora; defense},
language = {eng},
issn = {0006-2960},
journal = {Biochemistry},
title = {Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells},
volume = {44},
year = {2005}
}
TY - JOUR
ID - 570714
AU - Lochman, Jan - Kašparovský, Tomáš - Damborský, Jiří - Osman, Hanan - Ponchet, MIchel - Mikeš, Vladimír - Chaloupková, Radka
PY - 2005
TI - Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells
JF - Biochemistry
VL - 44
IS - 17
SP - 6565
EP - 6565
PB - USA
SN - 00062960
KW - Elicitor
KW - Phytophthora
KW - defense
N2 - We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobacco cells by these mutated proteins was proportional to their ability to bind sterols but not fatty acids. Although the cryptogein-sterol complex was suggested to be a form triggering a defense reaction in tobacco, some proteins unable to bind sterols induced the synthesis of active oxygen species and pH changes. The modeling experiments showed that conformational changes after the introduction of bulky residues into the loop of cryptogein resemble those induced by sterol binding. These changes may be necessary for the ability to trigger the early events by elicitins. However, the ability to stimulate necrosis in suspension tobacco cells and the expression of defense proteins in tobacco plants were linked neither to the lipid binding capacity nor to the capacity to provoke the early events. On the basis of these experiments and previous results, we propose that elicitins could stimulate two signal pathways. The first one induces necroses and the expression of pathogen-related proteins, includes tyrosine protein kinases and mitogen-activated protein kinases, and depends on the overall structure and charge distribution. The second type of interaction is mediated by phospholipase C and protein kinase C. It triggers the synthesis of active oxygen species and pH changes. This interaction depends on the ability of elicitins to bind sterols.
ER -
LOCHMAN, Jan, Tomáš KAŠPAROVSKÝ, Jiří DAMBORSKÝ, Hanan OSMAN, MIchel PONCHET, Vladimír MIKEŠ a Radka CHALOUPKOVÁ. Construction of Cryptogein Mutants, a Proteinaceous Elicitor from Phytophthora, with Altered Abilities To Induce a Defense Reaction in Tobacco Cells. \textit{Biochemistry}. USA, 2005, roč.~44, č.~17, s.~6565-6577. ISSN~0006-2960.
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