ŠMAJS, David, Petra MATĚJKOVÁ, SJ NORRIS a GM WEINSTOCK. Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků. In Sborník abstraktů XII. konference mladých mikrobiologů Tomáškovy dny 2003. 2003. vyd. Brno: Lékařská fakulta MU, 2003. s. 14 - 15, 1 s.
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Základní údaje
Originální název Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků
Název česky Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků
Název anglicky Transkriptome of Treponema pallidum: gene expression levels during experimental rabbit infection
Autoři ŠMAJS, David (203 Česká republika, garant), Petra MATĚJKOVÁ (203 Česká republika), SJ NORRIS (840 Spojené státy americké) a GM WEINSTOCK (840 Spojené státy americké).
Vydání 2003. vyd. Brno, Sborník abstraktů XII. konference mladých mikrobiologů Tomáškovy dny 2003, od s. 14 - 15, 1 s. 2003.
Nakladatel Lékařská fakulta MU
Další údaje
Originální jazyk čeština
Typ výsledku Stať ve sborníku
Obor Genetika a molekulární biologie
Stát vydavatele Estonsko
Utajení není předmětem státního či obchodního tajemství
Kód RIV RIV/00216224:14110/03:00012309
Organizační jednotka Lékařská fakulta
Klíčová slova anglicky transcriptome; treponema pallidum
Štítky transcriptome, Treponema pallidum
Změnil Změnil: prof. MUDr. David Šmajs, Ph.D., učo 1116. Změněno: 31. 5. 2005 10:31.
Anotace
DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from rabbit testes 10 days post infection. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.
Anotace anglicky
DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from rabbit testes 10 days post infection. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.
Návaznosti
NI7351, projekt VaVNázev: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice
Investor: Ministerstvo zdravotnictví ČR, Infekční choroby, mikrobiologie, epidemiologie a imunologie
VytisknoutZobrazeno: 17. 2. 2020 07:41