ŠMAJS, David, Petra MATĚJKOVÁ, SJ NORRIS and GM WEINSTOCK. Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků (Transkriptome of Treponema pallidum: gene expression levels during experimental rabbit infection). In Sborník abstraktů XII. konference mladých mikrobiologů Tomáškovy dny 2003. 2003. vyd. Brno: Lékařská fakulta MU, 2003. p. 14 - 15, 1 pp.
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Basic information
Original name Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků
Name in Czech Transkriptom spirochety Treponema pallidum: úroveň genové exprese během experimentální infekce králíků
Name (in English) Transkriptome of Treponema pallidum: gene expression levels during experimental rabbit infection
Authors ŠMAJS, David (203 Czech Republic, guarantor), Petra MATĚJKOVÁ (203 Czech Republic), SJ NORRIS (840 United States of America) and GM WEINSTOCK (840 United States of America).
Edition 2003. vyd. Brno, Sborník abstraktů XII. konference mladých mikrobiologů Tomáškovy dny 2003, p. 14 - 15, 1 pp. 2003.
Publisher Lékařská fakulta MU
Other information
Original language Czech
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Estonia
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/03:00012309
Organization unit Faculty of Medicine
Keywords in English transcriptome; treponema pallidum
Tags transcriptome, Treponema pallidum
Changed by Changed by: prof. MUDr. David Šmajs, Ph.D., učo 1116. Changed: 31/5/2005 10:31.
Abstract
DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from rabbit testes 10 days post infection. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.
Abstract (in English)
DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) isolated from rabbit testes 10 days post infection. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum (Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicating that the transcript levels generally corresponded to the relative protein concentrations. Genes with high transcript concentrations included those encoding flagellar filament and cytoplasmic filament proteins, prominent lipoproteins and membrane proteins, chaperonins, proteins involved in red-ox balance, chemotaxis regulatory proteins, a V-ATPase operon, and certain metabolic enzymes such as glycolytic pathway enzymes. Independent quantitation of the expression of 84 T. pallidum genes using real-time RT-PCR approach yielded a high degree of correlation (r = 0.94). Characterization of the T. pallidum transcriptome during experimental infection provides further insight into the importance of gene expression levels in the survival and pathogenesis of this bacterium in the mammalian host.
Links
NI7351, research and development projectName: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice
Investor: Ministry of Health of the CR, Infectious Diseases, Microbiology, Epidemiology and Immunology
PrintDisplayed: 3/4/2020 01:07