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@inproceedings{571832, author = {Novák, Petr and Žídek, Lukáš and Padrta, Petr and Sklenář, Vladimír}, address = {Brno}, booktitle = {19th NMR Valtice}, keywords = {NMR; Residual dipolar couplings; RNA; structure;}, language = {eng}, location = {Brno}, isbn = {80-210-3352-5}, pages = {30-30}, publisher = {Masarykova universita v Brně}, title = {Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings}, year = {2004} }
TY - JOUR ID - 571832 AU - Novák, Petr - Žídek, Lukáš - Padrta, Petr - Sklenář, Vladimír PY - 2004 TI - Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings PB - Masarykova universita v Brně CY - Brno SN - 8021033525 KW - NMR KW - Residual dipolar couplings KW - RNA KW - structure; N2 - The spliceosome is a complex of proteins and small nuclear RNAs (snRNAs) responsible for the removal of introns from pre-mRNA in eukaryotes. U6 snRNA has been demonstrated to be a vital element in splicing and its sequence is the most conserved of all snRNAs[1]. U6 snRNA from trypanosoma was our study. Determination of the solution structure of nucleic acid fragments is significantly improved if residual dipolar are measured. This data provides us additional long-range and local geometry restraints using a small degree of molecular alignment with the static magnetic field. We wanted to test polyethylenglycol (PEG) as a alignment medium for measuring the residual dipolar couplings (RDCs). PEG is a stable and unexpensive organic polymer that does not require a complex biochemical preparation like the most often used bacteriophage Pf1. In our study, applicability of PEG to several spin-state-selective E.COSY experiments was investigated. Measured RDCs were tested for internal consistency as refered in [2] in order to check the reliability of the values obtained in the PEG-aligned samples. ER -
NOVÁK, Petr, Lukáš ŽÍDEK, Petr PADRTA a Vladimír SKLENÁŘ. Refinement of Trypanosoma U6 RNA Stem-Loop Structure Using 1- and 2-Bond Residual Dipolar Couplings. In \textit{19th NMR Valtice}. Brno: Masarykova universita v Brně, 2004, s.~30-30. ISBN~80-210-3352-5.
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