2004
Liquid chromatographic-mass spectrometric determination of genistin and daidzin in soybean food samples after accelerated solvent extraction with modified content of extraction cell.
KLEJDUS, Bořivoj, Radka MIKELOVÁ, Vojtěch ADAM, Josef ZEHNÁLEK, Jan VACEK et. al.Základní údaje
Originální název
Liquid chromatographic-mass spectrometric determination of genistin and daidzin in soybean food samples after accelerated solvent extraction with modified content of extraction cell.
Název česky
HPLC/MS stanovení genistinu a daidzinu v potravinách na bázi sojových bobů
Název anglicky
Liquid chromatographic-mass spectrometric determination of genistin and daidzin in soybean food samples after accelerated solvent extraction with modified content of extraction cell.
Autoři
KLEJDUS, Bořivoj (203 Česká republika), Radka MIKELOVÁ (203 Česká republika), Vojtěch ADAM (203 Česká republika, garant), Josef ZEHNÁLEK (203 Česká republika), Jan VACEK (203 Česká republika), René KIZEK (203 Česká republika) a Vlastimil KUBÁŇ (203 Česká republika)
Vydání
Analytica Chimica Acta, 2004, 0003-2670
Další údaje
Jazyk
čeština
Typ výsledku
Článek v odborném periodiku
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.588
Kód RIV
RIV/00216224:14310/04:00011143
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000222494200001
Klíčová slova anglicky
accelerated solvent extraction; daidzin; foodstuffs; genistin; high-performance liquid chromatography; isoflavones; mass spectrometry; solvent extraction; Soxhlet extraction; soybean food; ultrasonic extraction; UV spectrophotometric detection
Štítky
Změněno: 22. 5. 2009 21:59, prof. RNDr. Vojtěch Adam, Ph.D.
V originále
A newly elaborated accelerated solvent extraction (ASE) procedure allowed more accurate (<2%) and precise (<5-8% rel.) HPLC/UV/MS determination of isoflavones in different kinds of food made from soybean (products of ecological agriculture), i.e. soy farina, soy meat granulate, soy meat laminae, soy meat bits, Protmix and EKO-B1. The on-column limits of detection (LOD) for daidzin/genistin were 1.2/1.6 fmol for the signal-to-noise ratio S/N = 3 and 1-3 fmol for daidzem, genistein, formononetin, biochanin A and ononin, respectively. Final concentrations of daidzin in soybean foods fluctuated between 30 and 60 mug/g of dry matter (DM). Significantly higher concentrations of genistin (between 60 and 130 mug/g DM) were found in the soybean foods.
Anglicky
A newly elaborated accelerated solvent extraction (ASE) procedure allowed more accurate (<2%) and precise (<5-8% rel.) HPLC/UV/MS determination of isoflavones in different kinds of food made from soybean (products of ecological agriculture), i.e. soy farina, soy meat granulate, soy meat laminae, soy meat bits, Protmix and EKO-B1. The on-column limits of detection (LOD) for daidzin/genistin were 1.2/1.6 fmol for the signal-to-noise ratio S/N = 3 and 1-3 fmol for daidzem, genistein, formononetin, biochanin A and ononin, respectively. Final concentrations of daidzin in soybean foods fluctuated between 30 and 60 mug/g of dry matter (DM). Significantly higher concentrations of genistin (between 60 and 130 mug/g DM) were found in the soybean foods.
Návaznosti
| MSM 432100001, záměr |
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