2004
Rapid isoflavone determination in soy plants (Glycine max) by high performance liquid chromatography coupled with diode-array detector.
MIKELOVÁ, Radka, Bořivoj KLEJDUS, Jitka PETRLOVÁ, David POTĚŠIL, Vojtěch ADAM et. al.Základní údaje
Originální název
Rapid isoflavone determination in soy plants (Glycine max) by high performance liquid chromatography coupled with diode-array detector.
Název česky
Stanovení isoflavonů u soji pomocí HPLC
Autoři
MIKELOVÁ, Radka (203 Česká republika, garant), Bořivoj KLEJDUS (203 Česká republika), Jitka PETRLOVÁ (203 Česká republika), David POTĚŠIL (203 Česká republika), Vojtěch ADAM (203 Česká republika), Marie STIBOROVÁ (203 Česká republika), Petr HODEK (203 Česká republika) a René KIZEK (203 Česká republika)
Vydání
Pardubice, Vitamins 2004 Targeted Nutritional Therapy. od s. 181-182, 292 s. 2004
Nakladatel
Univerzita Pardubice
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/04:00011151
Organizační jednotka
Přírodovědecká fakulta
ISBN
80-7194-644-3
Klíčová slova anglicky
HPLC; isoflaovne; SPE
Štítky
Změněno: 2. 8. 2005 11:15, prof. RNDr. Vojtěch Adam, Ph.D.
V originále
Phytoestrogens are nonsteroidal isoflavones present for example in soy foodstuffs. They have many health benefits, i.e. positively affect cardiovascular deceases and act as suppressor of reactive oxygen species. On the other hand they can also conduce to cancer reveal because their estrogen-like molecular structure. Many of the anticancer mechanisms of isoflavones were detected by in vitro procedures, including cell cultures. Several new extraction procedures, such as accelerated solvent extraction, have been developed as an improvement of the traditional procedures (soxhlet and/or ultrasonication methods) and also applied on isoflavones extraction from biological materials. High performance liquid chromatography (HPLC) with various detection methods is common for isoflavone determination. Fast column in HPLC enables shorter times of determination. The goal of our work was to apply ultra fast column on HPLC/DAD determination of isoflavones with modified accelerated solvent extraction (ASE).
Česky
Phytoestrogens are nonsteroidal isoflavones present for example in soy foodstuffs. They have many health benefits, i.e. positively affect cardiovascular deceases and act as suppressor of reactive oxygen species. On the other hand they can also conduce to cancer reveal because their estrogen-like molecular structure. Many of the anticancer mechanisms of isoflavones were detected by in vitro procedures, including cell cultures. Several new extraction procedures, such as accelerated solvent extraction, have been developed as an improvement of the traditional procedures (soxhlet and/or ultrasonication methods) and also applied on isoflavones extraction from biological materials. High performance liquid chromatography (HPLC) with various detection methods is common for isoflavone determination. Fast column in HPLC enables shorter times of determination. The goal of our work was to apply ultra fast column on HPLC/DAD determination of isoflavones with modified accelerated solvent extraction (ASE).
Návaznosti
| LN00A081, projekt VaV |
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