KLEJDUS, Bořivoj, Jitka PETRLOVÁ, David POTĚŠIL, Vojtěch ADAM, Radka MIKELOVÁ, Jan VACEK, René KIZEK a Vlastimil KUBÁŇ. Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. Analytica Chimica Acta. 2004, roč. 520, 1-2, s. 57-67. ISSN 0003-2670. |
Další formáty:
BibTeX
LaTeX
RIS
@article{571915, author = {KLEJDUS, Bořivoj and Petrlová, Jitka and Potěšil, David and Adam, Vojtěch and Mikelová, Radka and VACEK, Jan and Kizek, René and Kubáň, Vlastimil}, article_number = {1-2}, keywords = {diode array detection; drugs; fat-soluble vitamins; high-performance liquid chromatography; mass spectrometry; pharmaceutical preparations; simultaneous determination; vitamins; water-soluble vitamins}, language = {eng}, issn = {0003-2670}, journal = {Analytica Chimica Acta}, title = {Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.}, volume = {520}, year = {2004} }
TY - JOUR ID - 571915 AU - KLEJDUS, Bořivoj - Petrlová, Jitka - Potěšil, David - Adam, Vojtěch - Mikelová, Radka - VACEK, Jan - Kizek, René - Kubáň, Vlastimil PY - 2004 TI - Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. JF - Analytica Chimica Acta VL - 520 IS - 1-2 SP - 57-67 EP - 57-67 SN - 00032670 KW - diode array detection KW - drugs KW - fat-soluble vitamins KW - high-performance liquid chromatography KW - mass spectrometry KW - pharmaceutical preparations KW - simultaneous determination KW - vitamins KW - water-soluble vitamins N2 - Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values. ER -
KLEJDUS, Bořivoj, Jitka PETRLOVÁ, David POTĚŠIL, Vojtěch ADAM, Radka MIKELOVÁ, Jan VACEK, René KIZEK a Vlastimil KUBÁŇ. Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. \textit{Analytica Chimica Acta}. 2004, roč.~520, 1-2, s.~57-67. ISSN~0003-2670.
|