KLEJDUS, Bořivoj, Jitka PETRLOVÁ, David POTĚŠIL, Vojtěch ADAM, Radka MIKELOVÁ, Jan VACEK, René KIZEK a Vlastimil KUBÁŇ. Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. Analytica Chimica Acta. 2004, roč. 520, 1-2, s. 57-67. ISSN 0003-2670.
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Základní údaje
Originální název Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection.
Název česky Současné stanovení vitamínů rozpustných ve vodě a v tucích metodou HPLC
Autoři KLEJDUS, Bořivoj (203 Česká republika), Jitka PETRLOVÁ (203 Česká republika), David POTĚŠIL (203 Česká republika), Vojtěch ADAM (203 Česká republika, garant), Radka MIKELOVÁ (203 Česká republika), Jan VACEK (203 Česká republika), René KIZEK (203 Česká republika) a Vlastimil KUBÁŇ (203 Česká republika).
Vydání Analytica Chimica Acta, 2004, 0003-2670.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10406 Analytical chemistry
Stát vydavatele Česká republika
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 2.588
Kód RIV RIV/00216224:14310/04:00011152
Organizační jednotka Přírodovědecká fakulta
UT WoS 000223567500007
Klíčová slova anglicky diode array detection; drugs; fat-soluble vitamins; high-performance liquid chromatography; mass spectrometry; pharmaceutical preparations; simultaneous determination; vitamins; water-soluble vitamins
Štítky diode array detection, drugs, fat-soluble vitamins, high-performance liquid chromatography, mass spectrometry, pharmaceutical preparations, simultaneous determination, vitamins, water-soluble vitamins
Změnil Změnil: prof. RNDr. Vojtěch Adam, Ph.D., učo 63820. Změněno: 22. 5. 2009 22:04.
Anotace
Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values.
Anotace česky
Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150 mm x 4.6 mm, 3 mum particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010% trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7 ml min(-1). A linear gradient profile (A:B) started at 95:5 and was constant in the first 4 min, then linearly decreased up to 2:98 during the next 6 min, then it was constant in the next 20 min and finally linearly increased up to 95:5 ratio of water phase in the last 5 min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280 nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values.
Návaznosti
MSM 432100001, záměrNázev: Regulace biologických a technologických procesů pro konkurenceschopné zemědělství.
VytisknoutZobrazeno: 21. 6. 2024 01:39