| KLEJDUS, Bořivoj, Josef ZEHNÁLEK, Vojtěch ADAM, Jiří PETŘEK, René KIZEK, Jan VACEK, Libuše TRNKOVÁ, Roland ROZIK, Ladislav HAVEL a Vlastimil KUBÁŇ. Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium. Analytica Chimica Acta. 2004, roč. 520, č. 1, s. 117-124. ISSN 0003-2670. |
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@article{571928,
author = {KLEJDUS, Bořivoj and Zehnálek, Josef and Adam, Vojtěch and PETŘEK, Jiří and Kizek, René and VACEK, Jan and Trnková, Libuše and Rozik, Roland and Havel, Ladislav and Kubáň, Vlastimil},
article_number = {1},
keywords = {glutathione; HPLC/MS; voltammetry; Zea mays L.},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.},
volume = {520},
year = {2004}
}
TY - JOUR
ID - 571928
AU - KLEJDUS, Bořivoj - Zehnálek, Josef - Adam, Vojtěch - PETŘEK, Jiří - Kizek, René - VACEK, Jan - Trnková, Libuše - Rozik, Roland - Havel, Ladislav - Kubáň, Vlastimil
PY - 2004
TI - Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium.
JF - Analytica Chimica Acta
VL - 520
IS - 1
SP - 117-124
EP - 117-124
SN - 00032670
KW - glutathione
KW - HPLC/MS
KW - voltammetry
KW - Zea mays L.
N2 - Changes in fresh weight, overall protein amounts (Bradford method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for five days at three different cadmium concentrations (0, 10 and 100 uM CdCl2). A highly sensitive HPLC/MS for determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column (150 mm 2.1 mm, 3 um particle size) in an isocratic mode with acetonitrile - 0.01% TFA (5:95, flow rate 0.1 mL/min) was applied. Cadmium concentration was measured by differential pulse adsorptive stripping voltammetry (DPASV) after deposition on HMDE at potential --0.7 V (accumulation time 180 s, acetate buffer of pH 3.6) using an AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of a hanging mercury drop electrode (HMDE) as a working electrode with area 0.4 mm2, an Ag/AgCl/3 M KCl as a reference electrode and a Pt-wire as an auxiliary electrode. Maize kernels exposed to the highest cadmium concentration (100 uM) germinated formerly and much better. Rapid increase of fresh weight probably relates with more intensive uptake of water in order to decrease cadmium concentration. An intensive preservation of homeostasis of Cd ions in germinating plants by defending mechanisms might explain differences of uptake rate of cadmium. The linear increase of GSH content with exposure time at all studied concentration suggests the defending mechanisms might be triggered by concentrations of a heavy metal.
ER -
KLEJDUS, Bořivoj, Josef ZEHNÁLEK, Vojtěch ADAM, Jiří PETŘEK, René KIZEK, Jan VACEK, Libuše TRNKOVÁ, Roland ROZIK, Ladislav HAVEL a Vlastimil KUBÁŇ. Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutathione in the maize (Zea mays L.) kernels exposed to cadmium. \textit{Analytica Chimica Acta}. 2004, roč.~520, č.~1, s.~117-124. ISSN~0003-2670.
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