2004
The Use of RP-HPLC for Separation of Polycyclic Aromatic Nitrogen Heterocycles
ŠVÁBENSKÝ, Radim, Zdeněk ŠIMEK a Kamila KOČÍZákladní údaje
Originální název
The Use of RP-HPLC for Separation of Polycyclic Aromatic Nitrogen Heterocycles
Název česky
Použití RP-HPLC pro separaci polcyklyckých aromatických dusíkatých heterocyklů
Autoři
ŠVÁBENSKÝ, Radim (203 Česká republika), Zdeněk ŠIMEK (203 Česká republika, garant) a Kamila KOČÍ (203 Česká republika)
Vydání
Bordeaux, France, Abstracts-9th FECS Conference on Chemistry and the Environment, 2nd SFC meeting on Environmental Chemistry, od s. 216-216, 1 s. 2004
Nakladatel
9th FECS Conference on Chemistry and the Environment, 2nd SFC meeting on Environmental Chemistry
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10406 Analytical chemistry
Stát vydavatele
Francie
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/04:00025511
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
RP-HPLC ; Polycyclic Aromatic Nitrogen Heterocycles
Změněno: 29. 6. 2009 12:27, doc. RNDr. Zdeněk Šimek, CSc.
V originále
Polycyclic aromatic nitrogen heterocycles (PANH) are widely spread in the environment. They are released into environment from diverse sources such as incomplete combustion of fossil fuels, spills or effluents of several industrial activities, oil drilling, refining and storage, coal tar distillation and wood preservation. High performance liquid chromatography on reversed stationary phases is one of the most used analytical methods for separation of complex mixtures in environmental samples. The possibility to use liquid chromatography on octadecyl stationary phase for separation of polycyclic aromatic nitrogen heterocycles is demonstrated. Mixture of phosphate buffer/methanol was used as mobile phase in isocratic and gradient mode. Retention behaviour of mixture of PANH was studied at different chromatographic conditions. To describe retention changes of PANH as a function of acid-base properties, effect of different pH mobile phase in the region 2.0 to 6.0 have been investigated. Different concentration of phosphate buffer as component of mobile phase was used for ionic strength effect. Two detection techniques are compared, UV-DAD and fluorescence detector. The different elution sequences of individual compounds have been found on octadecylsilica stationary phases made by different manufacturers.
Česky
Polycyclic aromatic nitrogen heterocycles (PANH) are widely spread in the environment. They are released into environment from diverse sources such as incomplete combustion of fossil fuels, spills or effluents of several industrial activities, oil drilling, refining and storage, coal tar distillation and wood preservation. High performance liquid chromatography on reversed stationary phases is one of the most used analytical methods for separation of complex mixtures in environmental samples. The possibility to use liquid chromatography on octadecyl stationary phase for separation of polycyclic aromatic nitrogen heterocycles is demonstrated. Mixture of phosphate buffer/methanol was used as mobile phase in isocratic and gradient mode. Retention behaviour of mixture of PANH was studied at different chromatographic conditions. To describe retention changes of PANH as a function of acid-base properties, effect of different pH mobile phase in the region 2.0 to 6.0 have been investigated. Different concentration of phosphate buffer as component of mobile phase was used for ionic strength effect. Two detection techniques are compared, UV-DAD and fluorescence detector. The different elution sequences of individual compounds have been found on octadecylsilica stationary phases made by different manufacturers.
Návaznosti
| MSM 141100003, záměr |
|