Evaluation of (GTG)5-PCR fingerprinting for characterization of
enterococci isolated from bryndza cheese

a 2005

Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese

ŠVEC, Pavel; Marc VANCANNEYT; Milan SEMAN; Ivo SEDLÁČEK; Cindy SNAUWAERT et al.

Základní údaje

Originální název

Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese

Název česky

Vyhodnocení metody (GTG)5-PCR při charakterizaci enterokoků izolovaných z bryndzy.

Autoři

ŠVEC, Pavel; Marc VANCANNEYT; Milan SEMAN; Ivo SEDLÁČEK; Cindy SNAUWAERT; Karen LEFEBVRE a Jean SWINGS

Vydání

2nd International ASM-FEMS Conference on Enterococci, 2005

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/05:00013899

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Enterococcus; (GTG)5-PCR fingerprinting; bryndza

Štítky

(GTG)5-PCR fingerprinting, bryndza, Enterococcus

Změněno: 24. 3. 2010 13:40, doc. RNDr. Pavel Švec, Ph.D.

Anotace

ORIG CZ

V originále

A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E. faecium strains were separated into two clearly separated clusters (30 % similarity), but both these groups were confirmed as E. faecium by all methods mentioned above. No clear correlation between locality, sampling period and obtained fingerprint profiles was revealed. Our results imply that (GTG)5-PCR fingerprinting is useful for their identification at the species level, but not a suitable tool for epidemiological studies nor for typing of enterococcal strains. This work was supported by the Belgian Federal Science Policy Office by a research fellowship for P. S. obtained in the framework of the promotion of S&T cooperation with Central and Eastern Europe.

Česky

Práce se zabývá využitím PCR metody pro charakterizaci enterokoků izolovaných z bryndzy.

Návaznosti

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

ŠVEC, Pavel; Marc VANCANNEYT; Milan SEMAN; Ivo SEDLÁČEK; Cindy SNAUWAERT; Karen LEFEBVRE a Jean SWINGS. Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese. In 2nd International ASM-FEMS Conference on Enterococci. 2005.

@proceedings{575285,
   author = {Švec, Pavel and Vancanneyt, Marc and Seman, Milan and Sedláček, Ivo and Snauwaert, Cindy and Lefebvre, Karen and Swings, Jean},
   booktitle = {2nd International ASM-FEMS Conference on Enterococci},
   keywords = {Enterococcus; (GTG)5-PCR fingerprinting; bryndza},
   language = {eng},
   title = {Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese},
   year = {2005}
}

TY  - CONF
ID  - 575285
AU  - Švec, Pavel - Vancanneyt, Marc - Seman, Milan - Sedláček, Ivo - Snauwaert, Cindy - Lefebvre, Karen - Swings, Jean
PY  - 2005
TI  - Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese
KW  - Enterococcus
KW  - (GTG)5-PCR fingerprinting
KW  - bryndza
N2  - A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and 2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E. faecium strains were separated into two clearly separated clusters (30 % similarity), but both these groups were confirmed as E. faecium by all methods mentioned above. No clear correlation between locality, sampling period and obtained fingerprint profiles was revealed. Our results imply that (GTG)5-PCR fingerprinting is useful for their identification at the species level, but not a suitable tool for epidemiological studies nor for typing of enterococcal strains. This work was supported by the Belgian Federal Science Policy Office by a research fellowship for P. S. obtained in the framework of the promotion of S&T cooperation with Central and Eastern Europe.
ER  -

ŠVEC, Pavel; Marc VANCANNEYT; Milan SEMAN; Ivo SEDLÁČEK; Cindy SNAUWAERT; Karen LEFEBVRE a Jean SWINGS. Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese. In \textit{2nd International ASM-FEMS Conference on Enterococci}. 2005.

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