Genetic and molecular analysis of new sources of resistance to
powdery mildew in barley (Hordeum vulgare ssp. spontaneum)

D 2005

Genetic and molecular analysis of new sources of resistance to powdery mildew in barley (Hordeum vulgare ssp. spontaneum)

TETUROVÁ, Kateřina; Jana ŘEPKOVÁ; Radka PSOTKOVÁ; Pavel LÍZAL; Antonín DREISEITL et al.

Základní údaje

Originální název

Genetic and molecular analysis of new sources of resistance to powdery mildew in barley (Hordeum vulgare ssp. spontaneum)

Název česky

Genetická a molekulární analýza nových zdrojů odolnosti k padlí travnímu u ječmene (Hordeum vulgare ssp. spontaneum)

Autoři

TETUROVÁ, Kateřina; Jana ŘEPKOVÁ; Radka PSOTKOVÁ; Pavel LÍZAL a Antonín DREISEITL

Vydání

České Budějovice, Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005, s. 98-98, 2005

Nakladatel

Institute of Plant Molecular Biology ASCR

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14330/05:00012702

Organizační jednotka

Fakulta informatiky

ISBN

80-86778-16-9

Klíčová slova anglicky

Hordeum; powdery mildew; genetic mapping; microsatellites; resistance genes

Štítky

genetic mapping, hordeum, microsatellites, powdery mildew, resistance genes

Změněno: 31. 3. 2008 10:28, Ing. Kateřina Teturová, Ph.D.

Anotace

ORIG CZ

V originále

Thirteen newly identified resistance sources of wild barley Hordeum vulgare ssp. spontaneum to powdery mildew (fungal organism Blumeria graminis f. sp. hordei) were studied with the aim to identify the number of genes conferring resistance to powdery mildew, their allelic relationships, their allelism with Mla gene and the other aim was to localise individual genes on barley genetic map. The genetic as well as the molecular analyses were performed in the segregating F2 populations of crosses between winter barley cultivar Tiffany and individual resistance sources. In five resistance sources the resistance was found to be determined by one gene, in seven sources by two independent genes and in one source by three independent genes. The mode of inheritance of all analysed genes except one was determined to be dominant or semi-dominant, only one was recessive. Allelism tests confirmed that one resistance gene in all sources was Mla. Apparently, various alleles of Mla often participate in the genetic determination of this trait and the gene belongs to those with the highest efficiency. Our next interest was focused on the identification of new resistance genes. Therefore, two resistance accessions, PI466197 and PI466461, with two powdery mildew resistance genes were included in linkage recombination analysis in F2 generations by means of microsatellite DNA markers. Microsatellite markers were generated from barley genome maps and databases. So far amplification method has been optimized for 115 SSR markers. Polymorphism between the parents (H. spontaneum accessions vs. Tiffany) has been investigated with 90 SSR markers and analysed by agarose and polyacrylamide gel electrophoresis. The polymorphism of investigated sources ranged from 48 to 64%. Bulk segregant analysis was used for identification of markers linked with resistance genes. In PI466197, linkage was confirmed with Bmac0213 marker on the short arm of chromosome 1H (Mla locus) and Bmac0134 marker on the short arm of chromosome 2H. In PI466461, the resistance gene was linked with Bmag0021 marker on the short arm of chromosome 7H. The prospect of our work is the selection of progeny with fully effective resistance genes, single or in combinations, which is impossible with classical methods. Therefore, individual resistance genes have been mapped by means of DNA markers and tightly linked markers developed. The results can offer new possibilities in barley breeding.

Česky

Genetická a molekulární analýza byla provedena u 13 nově identifikovaných zdrojů odolnosti ječmene (Hordeum vulgare ssp. spontaneum) k padlí travnímu. Byl identifikován počet genů determinujících tento znak u jednotlivých zdrojů. Genetické mapování pomocí mikrosatelitových DNA markerů umožnilo lokalizovat jednotlivé geny a určit, zda se jedná o geny již známé nebo nově identifikované.

Návaznosti

GA522/03/0112, projekt VaV

Název: Lokalizace genů odolnosti k Blumeria graminis f. sp. hordei v nově zjištěných zdrojích Hordeum vulgare ssp. spontaneum pomocí molekulárních markerů

GD204/05/H505, projekt VaV

Název: Vývojová genetika rostlin

Investor: Grantová agentura ČR, Vývojová genetika rostlin

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

TETUROVÁ, Kateřina; Jana ŘEPKOVÁ; Radka PSOTKOVÁ; Pavel LÍZAL a Antonín DREISEITL. Genetic and molecular analysis of new sources of resistance to powdery mildew in barley (Hordeum vulgare ssp. spontaneum). In Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005. České Budějovice: Institute of Plant Molecular Biology ASCR, 2005, s. 98. ISBN 80-86778-16-9.

@inproceedings{583510,
   author = {Teturová, Kateřina and Řepková, Jana and Psotková, Radka and Lízal, Pavel and Dreiseitl, Antonín},
   address = {České Budějovice},
   booktitle = {Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005},
   keywords = {Hordeum; powdery mildew; genetic mapping; microsatellites; resistance genes},
   language = {eng},
   location = {České Budějovice},
   isbn = {80-86778-16-9},
   pages = {98-98},
   publisher = {Institute of Plant Molecular Biology ASCR},
   title = {Genetic and molecular analysis of new sources of resistance to powdery mildew in barley (Hordeum vulgare ssp. spontaneum)},
   year = {2005}
}

TY  - CONF
ID  - 583510
AU  - Teturová, Kateřina - Řepková, Jana - Psotková, Radka - Lízal, Pavel - Dreiseitl, Antonín
PY  - 2005
TI  - Genetic and molecular analysis of new sources of resistance to powdery mildew in barley (Hordeum vulgare ssp. spontaneum)
PB  - Institute of Plant Molecular Biology ASCR
CY  - České Budějovice
SN  - 8086778169
KW  - Hordeum
KW  - powdery mildew
KW  - genetic mapping
KW  - microsatellites
KW  - resistance genes
N2  - Thirteen newly identified resistance sources of wild barley Hordeum vulgare ssp. spontaneum to powdery mildew (fungal organism Blumeria graminis f. sp. hordei) were studied with the aim to identify the number of genes conferring resistance to powdery mildew, their allelic relationships, their allelism with Mla gene and the other aim was to localise individual genes on barley genetic map. The genetic as well as the molecular analyses were performed in the segregating F2 populations of crosses between winter barley cultivar Tiffany and individual resistance sources. In five resistance sources the resistance was found to be determined by one gene, in seven sources by two independent genes and in one source by three independent genes. The mode of inheritance of all analysed genes except one was determined to be dominant or semi-dominant, only one was recessive. Allelism tests confirmed that one resistance gene in all sources was Mla. Apparently, various alleles of Mla often participate in the genetic determination of this trait and the gene belongs to those with the highest efficiency. Our next interest was focused on the identification of new resistance genes. Therefore, two resistance accessions, PI466197 and PI466461, with two powdery mildew resistance genes were included in linkage recombination analysis in F2 generations by means of microsatellite DNA markers. Microsatellite markers were generated from barley genome maps and databases. So far amplification method has been optimized for 115 SSR markers. Polymorphism between the parents (H. spontaneum accessions vs. Tiffany) has been investigated with 90 SSR markers and analysed by agarose and polyacrylamide gel electrophoresis. The polymorphism of investigated sources ranged from 48 to 64%. Bulk segregant analysis was used for identification of markers linked with resistance genes. In PI466197, linkage was confirmed with Bmac0213 marker on the short arm of chromosome 1H (Mla locus) and Bmac0134 marker on the short arm of chromosome 2H. In PI466461, the resistance gene was linked with Bmag0021 marker on the short arm of chromosome 7H. The prospect of our work is the selection of progeny with fully effective resistance genes, single or in combinations, which is impossible with classical methods. Therefore, individual resistance genes have been mapped by means of DNA markers and tightly linked markers developed. The results can offer new possibilities in barley breeding.
ER  -

TETUROVÁ, Kateřina; Jana ŘEPKOVÁ; Radka PSOTKOVÁ; Pavel LÍZAL a Antonín DREISEITL. Genetic and molecular analysis of new sources of resistance to powdery mildew in barley (Hordeum vulgare ssp. spontaneum). In \textit{Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005}. České Budějovice: Institute of Plant Molecular Biology ASCR, 2005, s.~98. ISBN~80-86778-16-9.

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