2005
Addressing the role of vimentin in monocyte/macrophage differentiation using RNAi approach
MACEČKOVÁ, Vendula, Petr BENEŠ a Jan ŠMARDAZákladní údaje
Originální název
Addressing the role of vimentin in monocyte/macrophage differentiation using RNAi approach
Název česky
Výzkum úlohy vimentinu v monocytové/makrofágové diferenciační dráze založený na přístupu RNAi
Název anglicky
Addressing the role of vimentin in monocyte/macrophage differentiation using RNAi approach
Autoři
MACEČKOVÁ, Vendula (203 Česká republika), Petr BENEŠ (203 Česká republika) a Jan ŠMARDA (203 Česká republika, garant)
Vydání
České Budějovice, RNA club, s. 9-9, 2005
Nakladatel
Přírodovědecká fakulta, Univerzita Karlova v Praze
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
30200 3.2 Clinical medicine
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/05:00012709
Organizační jednotka
Přírodovědecká fakulta
ISBN
1214-8598
Klíčová slova anglicky
vimentin; differentiation; RNAi; phagocytosis
Štítky
Změněno: 21. 12. 2006 11:54, prof. RNDr. Jan Šmarda, CSc.
V originále
Vimentin is one of the IF proteins that undergo significant changes both in expression, structure and subcellular location during maturation of hematopoietic cells. The v-myb-transformed monoblasts of the cell line BM2 can be induced to differentiate towards macrophage-like cells using phorbol ester TPA and histon deacetylase inhibitor trichostatin A (TSA). We described earlier that level of vimentin increases during TPA- or TSA-induced differentiation of BM2 cells. In this study, RNAi technology was used to explore the role of vimentin in differentiation of BM2 cells. The cells were transfected with vector expressing siRNA targeted to endogenous vimentin mRNA and several independ clones of stable transfectants exhibiting 70% reduction of cellular vimentin level were obtained. The lack of vimentin altered morphology of the cells, reduced their phagocytic activity and production of ROS upon treatment with TPA or TSA. Our results demonstrate that up-regulation of vimentin is essential for formation of fully active macrophages during terminal steps of monocyte/macrophage differentiation.
Anglicky
Vimentin is one of the IF proteins that undergo significant changes both in expression, structure and subcellular location during maturation of hematopoietic cells. The v-myb-transformed monoblasts of the cell line BM2 can be induced to differentiate towards macrophage-like cells using phorbol ester TPA and histon deacetylase inhibitor trichostatin A (TSA). We described earlier that level of vimentin increases during TPA- or TSA-induced differentiation of BM2 cells. In this study, RNAi technology was used to explore the role of vimentin in differentiation of BM2 cells. The cells were transfected with vector expressing siRNA targeted to endogenous vimentin mRNA and several independ clones of stable transfectants exhibiting 70% reduction of cellular vimentin level were obtained. The lack of vimentin altered morphology of the cells, reduced their phagocytic activity and production of ROS upon treatment with TPA or TSA. Our results demonstrate that up-regulation of vimentin is essential for formation of fully active macrophages during terminal steps of monocyte/macrophage differentiation.
Návaznosti
| GA301/03/1055, projekt VaV |
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| GP301/03/D022, projekt VaV |
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| MSM0021622415, záměr |
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