KOSTLÁNOVÁ, Nikola, Edward MITCHELL, Nechama GILBOA-GARBER, Michaela WIMMEROVÁ and Anne IMBERTY. Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L. In Materials and Structure in Chemistry, Biology, Physics and Technology. ARS Print, Praha: Krystalografická společnost, R. Kužel, 2005, p. 148-148.
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Basic information
Original name Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
Name in Czech Náhled do strukturně funkčních vztahů lektinů bakterie Ralstonia solanacearum RSL, RS-IIL, RS20L
Name (in English) Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
Authors KOSTLÁNOVÁ, Nikola (203 Czech Republic), Edward MITCHELL (826 United Kingdom of Great Britain and Northern Ireland), Nechama GILBOA-GARBER (376 Israel), Michaela WIMMEROVÁ (203 Czech Republic, guarantor) and Anne IMBERTY (250 France).
Edition ARS Print, Praha, Materials and Structure in Chemistry, Biology, Physics and Technology, p. 148-148, 1 pp. 2005.
Publisher Krystalografická společnost, R. Kužel
Other information
Original language Czech
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/05:00014094
Organization unit Faculty of Science
Keywords in English lectin; crystallography; Ralstonia solanacearum
Tags CRYSTALLOGRAPHY, lectin, ralstonia solanacearum
Changed by Changed by: Mgr. Nikola Kostlánová, Ph.D., učo 12689. Changed: 3/11/2005 10:02.
Abstract
Lectins are a class of proteins of non-immune and non-enzymatic origin that bind carbohydrates specifically and reversibly. They express numerous biological activities, nearly all of which are based on their acting as recognition determinants in diverse biological processes including fertilization, pathogen-cell adhesion and recognition, inflammatory response and others. A number of pathogen microorganisms utilize lectin-carbohydrate interaction to recognized and infect host organism. The comprehension of the molecular mechanisms which gives a pathogenic bacterium the ability to invade, colonize and reorient the physiopathology of its host is a goal of primary importance and such studies may direct the conception of new strategies to fight these pathogenic agents1. Ralstonia solanacearum is soil-borne bacterium, which belongs to the group of beta-proteobacteria. It is responsible for bacterial wilts on more than 200 plant species including potato, tomato banana and others economically important corps 1. R. solanacearum, which is capable of living for prolonged periods in the soil, infects its hosts beginning with the root system and presents a very strong tropism for the xylem vessels. Its extensive multiplication in the water-conducting system leads to a systemic infection of the plant. This contribution describes three lectins RSL (9.9 kDa) 2, RS-IIL (11.6 kDa) 3 and RS20L (20 kDa) that have been found in R. solanacearum extract and purified using affinity chromatography. All lectins were crystallized by vapor diffusion and high and ultra-high (in case of 0.94Å resolution of RSL/-methyl fucose) resolution data were collected at ESRF, Grenoble, France. The structural data have been supplemented by ITC microcalorimetry, surface plasmon resonance studies and ELLA tests defining lectins specificity to carbohydrates including those, which are commonly present in nature and may be the target for the lectins in soil.
Abstract (in English)
Lectins are a class of proteins of non-immune and non-enzymatic origin that bind carbohydrates specifically and reversibly. They express numerous biological activities, nearly all of which are based on their acting as recognition determinants in diverse biological processes including fertilization, pathogen-cell adhesion and recognition, inflammatory response and others. A number of pathogen microorganisms utilize lectin-carbohydrate interaction to recognized and infect host organism. The comprehension of the molecular mechanisms which gives a pathogenic bacterium the ability to invade, colonize and reorient the physiopathology of its host is a goal of primary importance and such studies may direct the conception of new strategies to fight these pathogenic agents1. Ralstonia solanacearum is soil-borne bacterium, which belongs to the group of beta-proteobacteria. It is responsible for bacterial wilts on more than 200 plant species including potato, tomato banana and others economically important corps 1. R. solanacearum, which is capable of living for prolonged periods in the soil, infects its hosts beginning with the root system and presents a very strong tropism for the xylem vessels. Its extensive multiplication in the water-conducting system leads to a systemic infection of the plant. This contribution describes three lectins RSL (9.9 kDa) 2, RS-IIL (11.6 kDa) 3 and RS20L (20 kDa) that have been found in R. solanacearum extract and purified using affinity chromatography. All lectins were crystallized by vapor diffusion and high and ultra-high (in case of 0.94Å resolution of RSL/-methyl fucose) resolution data were collected at ESRF, Grenoble, France. The structural data have been supplemented by ITC microcalorimetry, surface plasmon resonance studies and ELLA tests defining lectins specificity to carbohydrates including those, which are commonly present in nature and may be the target for the lectins in soil.
Links
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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