2005
Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
KOSTLÁNOVÁ, Nikola, Edward MITCHELL, Nechama GILBOA-GARBER, Michaela WIMMEROVÁ, Anne IMBERTY et. al.Základní údaje
Originální název
Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
Název česky
Náhled do strukturně funkčních vztahů lektinů bakterie Ralstonia solanacearum RSL, RS-IIL, RS20L
Název anglicky
Insight into structure-function relationship of Ralstonia solanacearum lectins RSL, RS-IIL, RS20L
Autoři
KOSTLÁNOVÁ, Nikola (203 Česká republika), Edward MITCHELL (826 Velká Británie a Severní Irsko), Nechama GILBOA-GARBER (376 Izrael), Michaela WIMMEROVÁ (203 Česká republika, garant) a Anne IMBERTY (250 Francie)
Vydání
ARS Print, Praha, Materials and Structure in Chemistry, Biology, Physics and Technology, od s. 148-148, 1 s. 2005
Nakladatel
Krystalografická společnost, R. Kužel
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/05:00014094
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
lectin; crystallography; Ralstonia solanacearum
Štítky
Změněno: 3. 11. 2005 10:02, Mgr. Nikola Kostlánová, Ph.D.
V originále
Lectins are a class of proteins of non-immune and non-enzymatic origin that bind carbohydrates specifically and reversibly. They express numerous biological activities, nearly all of which are based on their acting as recognition determinants in diverse biological processes including fertilization, pathogen-cell adhesion and recognition, inflammatory response and others. A number of pathogen microorganisms utilize lectin-carbohydrate interaction to recognized and infect host organism. The comprehension of the molecular mechanisms which gives a pathogenic bacterium the ability to invade, colonize and reorient the physiopathology of its host is a goal of primary importance and such studies may direct the conception of new strategies to fight these pathogenic agents1. Ralstonia solanacearum is soil-borne bacterium, which belongs to the group of beta-proteobacteria. It is responsible for bacterial wilts on more than 200 plant species including potato, tomato banana and others economically important corps 1. R. solanacearum, which is capable of living for prolonged periods in the soil, infects its hosts beginning with the root system and presents a very strong tropism for the xylem vessels. Its extensive multiplication in the water-conducting system leads to a systemic infection of the plant. This contribution describes three lectins RSL (9.9 kDa) 2, RS-IIL (11.6 kDa) 3 and RS20L (20 kDa) that have been found in R. solanacearum extract and purified using affinity chromatography. All lectins were crystallized by vapor diffusion and high and ultra-high (in case of 0.94Å resolution of RSL/-methyl fucose) resolution data were collected at ESRF, Grenoble, France. The structural data have been supplemented by ITC microcalorimetry, surface plasmon resonance studies and ELLA tests defining lectins specificity to carbohydrates including those, which are commonly present in nature and may be the target for the lectins in soil.
Anglicky
Lectins are a class of proteins of non-immune and non-enzymatic origin that bind carbohydrates specifically and reversibly. They express numerous biological activities, nearly all of which are based on their acting as recognition determinants in diverse biological processes including fertilization, pathogen-cell adhesion and recognition, inflammatory response and others. A number of pathogen microorganisms utilize lectin-carbohydrate interaction to recognized and infect host organism. The comprehension of the molecular mechanisms which gives a pathogenic bacterium the ability to invade, colonize and reorient the physiopathology of its host is a goal of primary importance and such studies may direct the conception of new strategies to fight these pathogenic agents1. Ralstonia solanacearum is soil-borne bacterium, which belongs to the group of beta-proteobacteria. It is responsible for bacterial wilts on more than 200 plant species including potato, tomato banana and others economically important corps 1. R. solanacearum, which is capable of living for prolonged periods in the soil, infects its hosts beginning with the root system and presents a very strong tropism for the xylem vessels. Its extensive multiplication in the water-conducting system leads to a systemic infection of the plant. This contribution describes three lectins RSL (9.9 kDa) 2, RS-IIL (11.6 kDa) 3 and RS20L (20 kDa) that have been found in R. solanacearum extract and purified using affinity chromatography. All lectins were crystallized by vapor diffusion and high and ultra-high (in case of 0.94Å resolution of RSL/-methyl fucose) resolution data were collected at ESRF, Grenoble, France. The structural data have been supplemented by ITC microcalorimetry, surface plasmon resonance studies and ELLA tests defining lectins specificity to carbohydrates including those, which are commonly present in nature and may be the target for the lectins in soil.
Návaznosti
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