THE ASSOCIATION OF IL-6 GENE POLYMORPHISM  WITH ADHD IN
CHILDREN

D 2005

THE ASSOCIATION OF IL-6 GENE POLYMORPHISM WITH ADHD IN CHILDREN

ŠTAIF, Radim, Renáta PITELOVÁ, Pavel THEINER, Ivana DRTÍLKOVÁ, Omar ŠERÝ et. al.

Basic information

Original name

THE ASSOCIATION OF IL-6 GENE POLYMORPHISM WITH ADHD IN CHILDREN

Name in Czech

Asociace polymorfizmu genu pro IL-6 s ADHD u dětí

Authors

ŠTAIF, Radim (203 Czech Republic), Renáta PITELOVÁ (203 Czech Republic), Pavel THEINER (203 Czech Republic), Ivana DRTÍLKOVÁ (203 Czech Republic) and Omar ŠERÝ (203 Czech Republic, guarantor)

Edition

První vydání. Brno, IX. Pracovní setkání biochemiků a molekulárních biologů, p. 29-30, 2 pp. 2005

Publisher

Masarykova univerzita v Brně

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/05:00015217

Organization unit

Faculty of Science

ISBN

80-210-3635-4

Keywords in English

ADHD association IL-6 gene polymorphism

Změněno: 1/3/2007 17:10, prof. RNDr. Omar Šerý, Ph.D.

Abstract

ORIG CZ

V originále

Attention-deficit hyperactivity disorder (ADHD) is a common neurodevelopmental polygenetic heritable disorder that includes inattention, excessive motor activity, impulsivity and distractibility. In the past few years, interest in the molecular genetics of ADHD has grown enormously, with many groups searching for susceptibility genes. Dopaminergic genes have been prime candidates in the search for the genetic factors underlying novelty seeking because of the central role that dopamine plays in the brain's reward system. However, the findings are not conclusive and other new polymorphisms in different gene backgrounds are investigated. We have investigated whether there is an association between genes involving nervous system - cytokines, including IL-2, IL-6, and tumor necrosis factor-alpha (TNF-alpha). It is known that proinflammatory pleiotropic cytokines such as interleukin-6 (IL-6) are expressed in the central nervous system (CNS) during disease conditions and affect several brain functions including memory and learning. Genetic variation in the promoter region of the gene encoding the cytokine IL-6; -174 C/C polymorphism may encode enhanced production of IL-6. Our objective was to determine whether the CC genotype is associated with ADHD. In our association study, we studied the difference in genotype and allelic frequencies 174 polymorphism of IL-6 gene between hyperkinetic boys and boys in control group. We isolated genomic DNA from buccal tissue or blood sample collected from each individual. DNA was extracted by commercial kits UltraClean Tissue DNA or BloodSpin kit (Mobio, USA). Polymerase Chain Reaction (PCR) was used for determination of genotypes. PCR products were digested with restriction enzyme Taq I (MBI Fermentas) and subsequent electrophoresed on 2% agarose gel (Eliphore). The X2 test and Fischer-exact test were performed to assess the significance or non-significance association between studied groups of boys. We found the association between 174 polymorphism of IL-6 gene and ADHD. The allele C was observed frequently in ADHD group than in control group. These findings highlight the need for further investigations of cytokines genes, in additional independent ADHD samples, in the future.

In Czech

Zkoumali jsme vztah mezi ADHD a polymorfizmem genu pro IL-6 na souboru hyperkinetických chlapců. Zjistili jsme vztah mezi tímto polymorfizmem a ADHD.

Links

NF6520, research and development project

Name: Klinické a molekulárně-biologické koreláty hyperkinetické poruchy v dětském věku

Investor: Ministry of Health of the CR, Clinical and molecular-biological correlates of attention deficit hyperactivity disorder in children

Citovat

ŠTAIF, Radim, Renáta PITELOVÁ, Pavel THEINER, Ivana DRTÍLKOVÁ and Omar ŠERÝ. THE ASSOCIATION OF IL-6 GENE POLYMORPHISM WITH ADHD IN CHILDREN. In IX. Pracovní setkání biochemiků a molekulárních biologů. První vydání. Brno: Masarykova univerzita v Brně, 2005, p. 29-30. ISBN 80-210-3635-4.

@inproceedings{590305,
   author = {Štaif, Radim and Pitelová, Renáta and Theiner, Pavel and Drtílková, Ivana and Šerý, Omar},
   address = {Brno},
   booktitle = {IX. Pracovní setkání biochemiků a molekulárních biologů},
   edition = {První vydání},
   keywords = {ADHD association IL-6 gene polymorphism},
   language = {eng},
   location = {Brno},
   isbn = {80-210-3635-4},
   pages = {29-30},
   publisher = {Masarykova univerzita v Brně},
   title = {THE ASSOCIATION OF IL-6 GENE POLYMORPHISM WITH ADHD IN CHILDREN},
   year = {2005}
}

TY  - JOUR
ID  - 590305
AU  - Štaif, Radim - Pitelová, Renáta - Theiner, Pavel - Drtílková, Ivana - Šerý, Omar
PY  - 2005
TI  - THE ASSOCIATION OF IL-6 GENE POLYMORPHISM WITH ADHD IN CHILDREN
PB  - Masarykova univerzita v Brně
CY  - Brno
SN  - 8021036354
KW  - ADHD association IL-6 gene polymorphism
N2  - Attention-deficit hyperactivity disorder (ADHD) is a common neurodevelopmental polygenetic heritable disorder that includes inattention, excessive motor activity, impulsivity and distractibility. In the past few years, interest in the molecular genetics of ADHD has grown enormously, with many groups searching for susceptibility genes. Dopaminergic genes have been prime candidates in the search for the genetic factors underlying novelty seeking because of the central role that dopamine plays in the brain's reward system. However, the findings are not conclusive and other new polymorphisms in different gene backgrounds are investigated. We have investigated whether there is an association between genes involving nervous system - cytokines, including IL-2, IL-6, and tumor necrosis factor-alpha (TNF-alpha). It is known that proinflammatory pleiotropic cytokines such as interleukin-6 (IL-6) are expressed in the central nervous system (CNS) during disease conditions and affect several brain functions including memory and learning. Genetic variation in the promoter region of the gene encoding the cytokine IL-6; -174 C/C polymorphism may encode enhanced production of IL-6. Our objective was to determine whether the CC genotype is associated with ADHD. In our association study, we studied the difference in genotype and allelic frequencies 174 polymorphism of IL-6 gene between hyperkinetic boys and boys in control group. We isolated genomic DNA from buccal tissue or blood sample collected from each individual. DNA was extracted by commercial kits UltraClean Tissue DNA or BloodSpin kit (Mobio, USA). Polymerase Chain Reaction (PCR) was used for determination of genotypes. PCR products were digested with restriction enzyme Taq I (MBI Fermentas) and subsequent electrophoresed on 2% agarose gel (Eliphore). The X2 test and Fischer-exact test were performed to assess the significance or non-significance association between studied groups of boys. We found the association between 174 polymorphism of IL-6 gene and ADHD. The allele C was observed frequently in ADHD group than in control group. These findings highlight the need for further investigations of cytokines genes, in additional independent ADHD samples, in the future.
ER  -

ŠTAIF, Radim, Renáta PITELOVÁ, Pavel THEINER, Ivana DRTÍLKOVÁ and Omar ŠERÝ. THE ASSOCIATION OF IL-6 GENE POLYMORPHISM WITH ADHD IN CHILDREN. In \textit{IX. Pracovní setkání biochemiků a molekulárních biologů}. První vydání. Brno: Masarykova univerzita v Brně, 2005, p.~29-30. ISBN~80-210-3635-4.

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