Prevalence of vancomycin-resistant enterococci in hospitalized
patients and those living in the community in the Czech
Republic

D 2005

Prevalence of vancomycin-resistant enterococci in hospitalized patients and those living in the community in the Czech Republic

KOLÁŘ, Milan; Roman PANTŮČEK; Iva VÁGNEROVÁ; Michaela KESSELOVÁ; Pavel SAUER et al.

Základní údaje

Originální název

Prevalence of vancomycin-resistant enterococci in hospitalized patients and those living in the community in the Czech Republic

Název česky

Prevalence vankomycin-rezistentních enterokoků u hospitalizovaných pacientů a v komunitě v České republice

Autoři

KOLÁŘ, Milan; Roman PANTŮČEK ; Iva VÁGNEROVÁ; Michaela KESSELOVÁ; Pavel SAUER; Luboslava ČEKANOVÁ; Jiří DOŠKAŘ a Vladislava RŮŽIČKOVÁ

Vydání

Washington, D.C., USA, 45th annual ICAAC (Interscience Conference on Antimicrobial Agents and Chemotherapy), od s. 260-C2, 1 s. 2005

Nakladatel

American Society for Microbiology

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/05:00014187

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000238689700005

Klíčová slova anglicky

Enterococci; vancomycin resistance; Molecular epidemiology; VRE; gram-positive infections

Štítky

Enterococci, gram-positive infections, molecular epidemiology, vancomycin resistance, VRE

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2008 15:02, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

ORIG CZ

V originále

Background: The presented study aimed at determining the prevalence of vancomycin-resistant enterococci (VRE) in rectal swabs taken from both hospitalized patients and subjects from the community population in the Czech Republic. Methods: Between July 1, 2002 and July 1, 2003, rectal swabs were taken from hospitalized patients as well as individuals from the community to be utilized for isolating and identifying enterococci and their susceptibility to antibiotics. Vancomycin resistance phenotypes were verified by PCR detection of vanA, vanB, vanC1 and vanC2 genes. A molecular biology analysis was performed in Enterococcus faecium VanA strains. To determine the relationship of strains, macrorestriction analysis of the total chromosomal DNA digested with SmaI restriction endonuclease was used. Results: During the observed period, 2,157 rectal swabs from the hospitalized patients and 4,874 rectal swabs from the subjects in community setting were examined. In total, 27 VRE of hospital origin and 13 community-population strains were isolated. The prevalence of VRE in the gastrointestinal tract was 2.3% in the hospitalized patients and 0.6% in the community subjects. The prevailing strains were Enterococcus faecium VanA (70.4%) in the VRE of hospital origin and Enterococcus gallinarum VanC (46.2%) in the community VRE. Mutual comparison between the hospital and community Enterococcus faecium VanA strains showed no similarity. Conclusions: In the Czech Republic, VRE were proved both in community and hospital settings. Their prevalence in rectal swabs is low and does not exceed the values reported in other European countries.

Česky

neuvedeno

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

KOLÁŘ, Milan; Roman PANTŮČEK; Iva VÁGNEROVÁ; Michaela KESSELOVÁ; Pavel SAUER; Luboslava ČEKANOVÁ; Jiří DOŠKAŘ a Vladislava RŮŽIČKOVÁ. Prevalence of vancomycin-resistant enterococci in hospitalized patients and those living in the community in the Czech Republic. In 45th annual ICAAC (Interscience Conference on Antimicrobial Agents and Chemotherapy). Washington, D.C., USA: American Society for Microbiology, 2005, s. 260-C2, 1 s.

@inproceedings{590964,
   author = {Kolář, Milan and Pantůček, Roman and Vágnerová, Iva and Kesselová, Michaela and Sauer, Pavel and Čekanová, Luboslava and Doškař, Jiří and Růžičková, Vladislava},
   address = {Washington, D.C., USA},
   booktitle = {45th annual ICAAC (Interscience Conference on Antimicrobial Agents and Chemotherapy)},
   keywords = {Enterococci; vancomycin resistance; Molecular epidemiology; VRE; gram-positive infections},
   language = {eng},
   location = {Washington, D.C., USA},
   pages = {260-C2},
   publisher = {American Society for Microbiology},
   title = {Prevalence of vancomycin-resistant enterococci in hospitalized patients and those living in the community in the Czech Republic},
   url = {http://www.icaac.org/45ICAAC/default.asp},
   year = {2005}
}

TY  - CONF
ID  - 590964
AU  - Kolář, Milan - Pantůček, Roman - Vágnerová, Iva - Kesselová, Michaela - Sauer, Pavel - Čekanová, Luboslava - Doškař, Jiří - Růžičková, Vladislava
PY  - 2005
TI  - Prevalence of vancomycin-resistant enterococci in hospitalized patients and those living in the community in the Czech Republic
PB  - American Society for Microbiology
CY  - Washington, D.C., USA
KW  - Enterococci
KW  - vancomycin resistance
KW  - Molecular epidemiology
KW  - VRE
KW  - gram-positive infections
UR  - http://www.icaac.org/45ICAAC/default.asp
N2  - Background: The presented study aimed at determining the prevalence of vancomycin-resistant enterococci (VRE) in rectal swabs taken from both hospitalized patients and subjects from the community population in the Czech Republic. Methods: Between July 1, 2002 and July 1, 2003, rectal swabs were taken from hospitalized patients as well as individuals from the community to be utilized for isolating and identifying enterococci and their susceptibility to antibiotics. Vancomycin resistance phenotypes were verified by PCR detection of vanA, vanB, vanC1 and vanC2 genes. A molecular biology analysis was performed in Enterococcus faecium VanA strains. To determine the relationship of strains, macrorestriction analysis of the total chromosomal DNA digested with SmaI restriction endonuclease was used. Results: During the observed period, 2,157 rectal swabs from the hospitalized patients and 4,874 rectal swabs from the subjects in community setting were examined. In total, 27 VRE of hospital origin and 13 community-population strains were isolated. The prevalence of VRE in the gastrointestinal tract was 2.3% in the hospitalized patients and 0.6% in the community subjects. The prevailing strains were Enterococcus faecium VanA (70.4%) in the VRE of hospital origin and Enterococcus gallinarum VanC (46.2%) in the community VRE. Mutual comparison between the hospital and community Enterococcus faecium VanA strains showed no similarity. Conclusions: In the Czech Republic, VRE were proved both in community and hospital settings. Their prevalence in rectal swabs is low and does not exceed the values reported in other European countries.
ER  -

KOLÁŘ, Milan; Roman PANTŮČEK; Iva VÁGNEROVÁ; Michaela KESSELOVÁ; Pavel SAUER; Luboslava ČEKANOVÁ; Jiří DOŠKAŘ a Vladislava RŮŽIČKOVÁ. Prevalence of vancomycin-resistant enterococci in hospitalized patients and those living in the community in the Czech Republic. In \textit{45th annual ICAAC (Interscience Conference on Antimicrobial Agents and Chemotherapy)}. Washington, D.C., USA: American Society for Microbiology, 2005, s.~260-C2, 1 s.

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