| DUBOVÝ, Petr, Radim JANČÁLEK a Ilona KLUSÁKOVÁ. A heterogeneous immunofluorescence staining for laminin-1 and related basal lamina molecules in the dorsal root ganglia following constriction nerve injury. Histochem Cell Biol. Springer-Verlag GmbH, 2005, roč. 125, č. 6, s. 671-680. ISSN 0948-6143. |
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@article{602225,
author = {Dubový, Petr and Jančálek, Radim and Klusáková, Ilona},
article_number = {6},
keywords = {satellite glial cells; type IV collagen; nidogen},
language = {eng},
issn = {0948-6143},
journal = {Histochem Cell Biol},
title = {A heterogeneous immunofluorescence staining for laminin-1 and related basal lamina molecules in the dorsal root ganglia following constriction nerve injury},
volume = {125},
year = {2005}
}
TY - JOUR
ID - 602225
AU - Dubový, Petr - Jančálek, Radim - Klusáková, Ilona
PY - 2005
TI - A heterogeneous immunofluorescence staining for laminin-1 and related basal lamina molecules in the dorsal root ganglia following constriction nerve injury
JF - Histochem Cell Biol
VL - 125
IS - 6
SP - 671-680
EP - 671-680
PB - Springer-Verlag GmbH
SN - 09486143
KW - satellite glial cells
KW - type IV collagen
KW - nidogen
N2 - The bodies of primary sensory neurons and their satellite glial cells (SGCs) are limited by the basal laminae from extracellular matrix of the dorsal rot ganglia (DRG). The basal laminae displayed uniform immunofluorescence staining for laminin-1 in the sections of rat intact (naive) DRG. A proximal or distal ligature of the spinal nerves resulted in a heterogeneous immunostaining for laminin-1 around neuron-SGC units in the sections of corresponding DRG. The pattern of irregular laminin-1 immunofluorescence was more extensive in the ipsilateral than contralateral DRG of the operated rats. The immunofluorescence for laminin-1 exactly coincided with binding of Concanavalin-A as well as immunostaining for type IV collagen in both naive DRG and DRG affected by nerve ligature. Nidogen immunostaining decreased or fully disappeared at the surface of SGCs consistently with immunofluorescence staining for laminin-1, but retained or increased in the endothelial cells and ED-1 positive cells invaded the DRG affected by nerve ligature. The results indicate an alteration of the content of basal laminae surrounding the bodies of primary sensory neurons and their SGSs following nerve constriction injury. A modulation of the basal laminae may be related with other cellular and molecular alterations related with peripheral neuropathic pain, e.g., expansion of sympathetic sprouts.
ER -
DUBOVÝ, Petr, Radim JANČÁLEK a Ilona KLUSÁKOVÁ. A heterogeneous immunofluorescence staining for laminin-1 and related basal lamina molecules in the dorsal root ganglia following constriction nerve injury. \textit{Histochem Cell Biol}. Springer-Verlag GmbH, 2005, roč.~125, č.~6, s.~671-680. ISSN~0948-6143.
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