| PACHERNÍK, J., V. BRYJA, M. EŠNER, L. KUBALA, Petr DVOŘÁK a Aleš HAMPL. Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum. Physiol. Res. 2005, roč. 54, č. 1, s. 115-122. ISSN 0862-8408. |
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@article{609168,
author = {Pacherník, J. and Bryja, V. and Ešner, M. and Kubala, L. and Dvořák, Petr and Hampl, Aleš},
article_number = {1},
keywords = {cells},
language = {eng},
issn = {0862-8408},
journal = {Physiol. Res.},
title = {Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum},
volume = {54},
year = {2005}
}
TY - JOUR
ID - 609168
AU - Pacherník, J. - Bryja, V. - Ešner, M. - Kubala, L. - Dvořák, Petr - Hampl, Aleš
PY - 2005
TI - Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum
JF - Physiol. Res.
VL - 54
IS - 1
SP - 115-122
EP - 115-122
SN - 08628408
KW - cells
N2 - This study employs mouse embryonal carcinoma (EC) cells P19 to investigate the effects of serum on RA-induced neural differentiation occurring in a simplified monolayer culture. The data show that mouse EC cells are able to produce neural cells upon exposure to RA even without culture in three-dimensional embryoid bodies (EBs). However, in contrast to standard EBs-involving protocol(s), neural differentiation in monolayer only takes place when complex signaling from serum factors is avoided. This simple and efficient strategy is proposed to serve as a basis for neurodifferentiation studies in vitro.
ER -
PACHERNÍK, J., V. BRYJA, M. EŠNER, L. KUBALA, Petr DVOŘÁK a Aleš HAMPL. Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum. \textit{Physiol. Res.}. 2005, roč.~54, č.~1, s.~115-122. ISSN~0862-8408.
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