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@article{617344,
author = {Havliš, Jan and Štosová, Tatiana and Šebela, Marek and Lenobl, René},
article_location = {Praha},
article_number = {12},
keywords = {TANDEM MASS-SPECTROMETRY; AMINO-ACID SEQUENCE; PROTEIN IDENTIFICATION; POLYACRYLAMIDE-GELS; QUADRUPOLE-TIME; LYSOBACTER-ENZYMOGENES; CHEMICAL-MODIFICATION; CARBOXYPEPTIDASE-Y; PSEUDOMONAS-FRAGI; MEMBRANE-PROTEINS},
language = {cze},
issn = {0009-2770},
journal = {Chemické listy},
title = {Proteolytické enzymy: význam pro proteomiku},
volume = {99},
year = {2005}
}
TY - JOUR
ID - 617344
AU - Havliš, Jan - Štosová, Tatiana - Šebela, Marek - Lenobl, René
PY - 2005
TI - Proteolytické enzymy: význam pro proteomiku
JF - Chemické listy
VL - 99
IS - 12
SP - 896-905
EP - 896-905
PB - Czech Chemical Society
SN - 00092770
KW - TANDEM MASS-SPECTROMETRY
KW - AMINO-ACID SEQUENCE
KW - PROTEIN IDENTIFICATION
KW - POLYACRYLAMIDE-GELS
KW - QUADRUPOLE-TIME
KW - LYSOBACTER-ENZYMOGENES
KW - CHEMICAL-MODIFICATION
KW - CARBOXYPEPTIDASE-Y
KW - PSEUDOMONAS-FRAGI
KW - MEMBRANE-PROTEINS
N2 - Proteolytic enzymes (peptidases) are important tools for proteomic research. This article reviews their application to digestion of proteins, which commonly precedes the mass spectrometry analysis of proteins. In the opening part, mechanism of proteolysis is described together with a detailed introduction to classification and nomenclature of enzymes. The following part brings an overview of proteolytic enzymes that are currently used in expression and functional proteomics including their properties and specificity. The review continues with reports on assays of proteolytic activity and computer prediction of peptide cleavage. The methodology of in-solution and in-gel digestion of samples and the use of peptidases for cleavage of proteins on membrane surfaces is also mentioned. Furthermore, chemical modifications of peptidases for improving their stability to autolysis and thermal inactivation are documented. Peptidase treatment of hydrophobic proteins in solutions containing organic solvents are also mentioned. Finally, new perspectives of proteolysis in shotgun proteomics are outlined.
ER -
HAVLIŠ, Jan, Tatiana ŠTOSOVÁ, Marek ŠEBELA a René LENOBL. Proteolytické enzymy: význam pro proteomiku. \textit{Chemické listy}. Praha: Czech Chemical Society, 2005, roč.~99, č.~12, s.~896-905. ISSN~0009-2770.
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