New model for evaluation of endocrine disruption of steroidogenesis in H295R cell line
HILSCHEROVÁ, Klára, Jiří NOVÁK a John Paul GIESY. New model for evaluation of endocrine disruption of steroidogenesis in H295R cell line. In HILSCHEROVÁ, Klára a John Paul GIESY. ECOTOX 2005. Brno: Brno : Masaryk University, 2005., 2005, s. 29. ISBN 8021037997. |
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Základní údaje | |
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Originální název | New model for evaluation of endocrine disruption of steroidogenesis in H295R cell line |
Název česky | Nový model pro hodnocení endokrinní disrupce steroidogeneze v buněčné linii H295R |
Autoři | HILSCHEROVÁ, Klára (203 Česká republika), Jiří NOVÁK (203 Česká republika, garant) a John Paul GIESY (840 Spojené státy). |
Vydání | Brno, ECOTOX 2005, s. 29-29, 2005. |
Nakladatel | Brno : Masaryk University, 2005. |
Další údaje | |
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Originální jazyk | angličtina |
Typ výsledku | Stať ve sborníku |
Obor | 30304 Public and environmental health |
Stát vydavatele | Česká republika |
Utajení | není předmětem státního či obchodního tajemství |
Kód RIV | RIV/00216224:14310/05:00014599 |
Organizační jednotka | Přírodovědecká fakulta |
ISBN | 8021037997 |
Klíčová slova anglicky | steroidogenesis H295R disruption |
Štítky | steroidogenesis H295R disruption |
Změnil | Změnila: doc. Mgr. Klára Hilscherová, Ph.D., učo 133960. Změněno: 25. 6. 2007 21:56. |
Anotace |
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One of the most hazardous properties of xenobiotics is their ability to interact with endocrine system of the organisms leading to number of negative consequences. After discovery of endocrine disrupting potential of wide range of environmental pollutants, the main concern was devoted to study of mechanism of interaction between xenobiotic compounds and steroid hormone receptors (particularly interaction with estrogenic receptor). Less attention was focused on other parts of this signaling pathway, such as steroidogenesis, which is involved in steroid signaling pathway that regulates many crucial physiologic processes such as carcinogenesis, immunotoxicity or reduced fecundity. We have developed a model for assessment of the potency of various chemicals or their mixtures (pharmaceuticals, environmental contaminants, pesticides...) to affect levels of produced steroids. The model is based on a human carcinoma cell line H295R, which retains the ability to synthesize most of the important steroidogenic enzymes. The cells were cultivated in a medium with charcoal-dextrane treated serum. After 24-hour exposure to the tested chemicals and forskolin the levels of steroids were measured directly in cultivation medium using ELISA kits. The results were normalized to total protein content. We studied endocrine disrupting effects of several xenobiotics (e.g. POPs: TCDD, B(a)P; pesticides: vinclozolin; pharmaceuticals: metyrapone, spironolactone) on production of number of steroid hormones (such as testosterone, 17b-estradiol, androstenedione, 17-OH-progesterone, cortisol). Obtained results show that the effects of the chemicals are compound-specific and that the chemicals can interact with multiple levels of steroidogenesis. For example, incubation of cells with TCDD shows dose dependent increase in production of testosterone and 17b-estradiol while production of androstenedione and 17-OH-progesterone stays unchanged. The model used does not allow detailed mechanistic studies of the effects of chemicals on steroidogenesis at molecular level (like real time PCR or enzyme activity studies). On the other hand, it shows the final effects on the level of production of hormones that is more toxicologically relevant than outputs from methods mentioned above. The assay is suitable for evaluating of endocrine disrupting effects of chemicals and for screening purposes thanks to its relatively simplicity and low costs. |
Anotace česky |
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One of the most hazardous properties of xenobiotics is their ability to interact with endocrine system of the organisms leading to number of negative consequences. After discovery of endocrine disrupting potential of wide range of environmental pollutants, the main concern was devoted to study of mechanism of interaction between xenobiotic compounds and steroid hormone receptors (particularly interaction with estrogenic receptor). Less attention was focused on other parts of this signaling pathway, such as steroidogenesis, which is involved in steroid signaling pathway that regulates many crucial physiologic processes such as carcinogenesis, immunotoxicity or reduced fecundity. We have developed a model for assessment of the potency of various chemicals or their mixtures (pharmaceuticals, environmental contaminants, pesticides...) to affect levels of produced steroids. The model is based on a human carcinoma cell line H295R, which retains the ability to synthesize most of the important steroidogenic enzymes. The cells were cultivated in a medium with charcoal-dextrane treated serum. After 24-hour exposure to the tested chemicals and forskolin the levels of steroids were measured directly in cultivation medium using ELISA kits. The results were normalized to total protein content. We studied endocrine disrupting effects of several xenobiotics (e.g. POPs: TCDD, B(a)P; pesticides: vinclozolin; pharmaceuticals: metyrapone, spironolactone) on production of number of steroid hormones (such as testosterone, 17b-estradiol, androstenedione, 17-OH-progesterone, cortisol). Obtained results show that the effects of the chemicals are compound-specific and that the chemicals can interact with multiple levels of steroidogenesis. For example, incubation of cells with TCDD shows dose dependent increase in production of testosterone and 17b-estradiol while production of androstenedione and 17-OH-progesterone stays unchanged. The model used does not allow detailed mechanistic studies of the effects of chemicals on steroidogenesis at molecular level (like real time PCR or enzyme activity studies). On the other hand, it shows the final effects on the level of production of hormones that is more toxicologically relevant than outputs from methods mentioned above. The assay is suitable for evaluating of endocrine disrupting effects of chemicals and for screening purposes thanks to its relatively simplicity and low costs. |
Návaznosti | |
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GP525/05/P160, projekt VaV | Název: In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů |
Investor: Grantová agentura ČR, In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů | |
MSM0021622412, záměr | Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL) |
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni |
VytisknoutZobrazeno: 30. 5. 2024 08:00