Genotoxic activity of a technical toxaphene mixture and its
photodegradation products  in the SOS genotoxicity tests

J 2005

Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests

BARTOŠ, Tomáš; Michal ŠKAREK; Pavel ČUPR; Petra KOSUBOVÁ; Ivan HOLOUBEK et al.

Základní údaje

Originální název

Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests

Název česky

Genotoxický potenciál toxafenu a jeho fotodegradačních produktů

Autoři

BARTOŠ, Tomáš; Michal ŠKAREK; Pavel ČUPR; Petra KOSUBOVÁ a Ivan HOLOUBEK

Vydání

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, Amsterdam, Netherlands, ELSEVIER SCIENCE BV, 2005, 1383-5718

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30304 Public and environmental health

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.188

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/05:00013140

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000226764600002

Klíčová slova anglicky

Toxaphene; genotoxicity assays; SOS response; umuC test; photodegradation; UV-irradiation

Štítky

genotoxicity assays, Photodegradation, SOS response, toxaphene, umuC test, UV-irradiation

Změněno: 11. 5. 2007 09:21, RNDr. Tomáš Bartoš, Ph.D.

Anotace

ORIG CZ

V originále

Toxaphene (CAS No. 800-35-2) is a complex mixture of several hundred components, that was used worldwide primarily as an agricultural pesticide with insecticide effects in the second half of the 20th century. In vitro investigations of genotoxicity and mutagenicity of toxaphene were in literature generally described, but they provided somewhat equivocal results. We re-evaluated the technical toxaphene in two prokaryotic systems. The SOS Chromotest showed high sensitivity to toxaphene. Three highest concentrations (40, 20 and 10 mg/l) were clearly positive and the dose-response effect was evident. In the umuC assay at concentrations from 2.5 to 40.0 mg/l, a dose-dependent increase in genotoxic activity was observed, but these results were found to be insignificant. Genotoxicity of toxaphene and its photodegradation products was also examined in this study after UV irradiation (3-6-9 h) at concentrations from 7.5 to 60.0 mg/l. Irradiated solution of technical toxaphene after 3 h showed no significant evidence of growth inhibition. However exposure of Salmonella to 6-h UV-irradiated toxaphene showed toxic effect compared to negative control. After 9-h irradiation rapid decrease of bacterial growth was observed. Activity of grbeta-galactosidase of toxaphene solution was markedly increased after 6 and 9 h irradiation and significantly reached 2.4-times, respectively 3.1-times higher values relative to control variant and exceeded the criteria of a significant genotoxicity. These results show that while technical toxaphene is a weak, direct-acting mutagen in some bacterial tests, the evidence of dose-dependent toxicity and genotoxicity its photoproducts could be conclusively demonstrated by umuC testing procedure.

Česky

Toxaphene

Návaznosti

GA525/03/0367, projekt VaV

Název: Ekotoxikologie persistentních organických polutantů životního prostředí

Investor: Grantová agentura ČR, Ekotoxikologie persistentních organických polutantů životního prostředí

Citovat

BARTOŠ, Tomáš; Michal ŠKAREK; Pavel ČUPR; Petra KOSUBOVÁ a Ivan HOLOUBEK. Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. Amsterdam, Netherlands: ELSEVIER SCIENCE BV, 2005, roč. 2005, č. 565, s. 113-120. ISSN 1383-5718.

@article{618518,
   author = {Bartoš, Tomáš and Škarek, Michal and Čupr, Pavel and Kosubová, Petra and Holoubek, Ivan},
   article_location = {Amsterdam, Netherlands},
   article_number = {565},
   keywords = {Toxaphene; genotoxicity assays; SOS response; umuC test; photodegradation; UV-irradiation},
   language = {eng},
   issn = {1383-5718},
   journal = {MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS},
   title = {Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests},
   volume = {2005},
   year = {2005}
}

TY  - JOUR
ID  - 618518
AU  - Bartoš, Tomáš - Škarek, Michal - Čupr, Pavel - Kosubová, Petra - Holoubek, Ivan
PY  - 2005
TI  - Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests
JF  - MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
VL  - 2005
IS  - 565
SP  - 113-120
EP  - 113-120
PB  - ELSEVIER SCIENCE BV
SN  - 13835718
KW  - Toxaphene
KW  - genotoxicity assays
KW  - SOS response
KW  - umuC test
KW  - photodegradation
KW  - UV-irradiation
N2  - Toxaphene (CAS No. 800-35-2) is a complex mixture of several hundred components, that was used worldwide primarily as an agricultural pesticide with insecticide effects in the second half of the 20th century. In vitro investigations of genotoxicity and mutagenicity of toxaphene were in literature generally described, but they provided somewhat equivocal results. We re-evaluated the technical toxaphene in two prokaryotic systems. The SOS Chromotest showed high sensitivity to toxaphene. Three highest concentrations (40, 20 and 10 mg/l) were clearly positive and the dose-response effect was evident. In the umuC assay at concentrations from 2.5 to 40.0 mg/l, a dose-dependent increase in genotoxic activity was observed, but these results were found to be insignificant. Genotoxicity of toxaphene and its photodegradation products was also examined in this study after UV irradiation (3-6-9 h) at concentrations from 7.5 to 60.0 mg/l. Irradiated solution of technical toxaphene after 3 h showed no significant evidence of growth inhibition. However exposure of Salmonella to 6-h UV-irradiated toxaphene showed toxic effect compared to negative control. After 9-h irradiation rapid decrease of bacterial growth was observed. Activity of grbeta-galactosidase of toxaphene solution was markedly increased after 6 and 9 h irradiation and significantly reached 2.4-times, respectively 3.1-times higher values relative to control variant and exceeded the criteria of a significant genotoxicity. These results show that while technical toxaphene is a weak, direct-acting mutagen in some bacterial tests, the evidence of dose-dependent toxicity and genotoxicity its photoproducts could be conclusively demonstrated by umuC testing procedure.
ER  -

BARTOŠ, Tomáš; Michal ŠKAREK; Pavel ČUPR; Petra KOSUBOVÁ a Ivan HOLOUBEK. Genotoxic activity of a technical toxaphene mixture and its photodegradation products in the SOS genotoxicity tests. \textit{MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS}. Amsterdam, Netherlands: ELSEVIER SCIENCE BV, 2005, roč.~2005, č.~565, s.~113-120. ISSN~1383-5718.

Přehled o publikaci