The yield of chromosomal aberrations in children with acute
leukemia using methods GTG banding, FISH, CGH and HR-CGH

D 2005

The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH

MENTZLOVÁ, Dita, Alexandra OLTOVÁ, Vlasta LINKOVÁ, Petr KUGLÍK, Jaroslav ŠTĚRBA et. al.

Základní údaje

Originální název

The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH

Název česky

Chromozomové aberace u dětí s akutní leukémií studované pomocí technik G-pruhování, fluorescenční hybridizace in situ, komparativní genomové hybridizace a komparativní genomové hybridizace s vysokým rozlišením

Autoři

MENTZLOVÁ, Dita (203 Česká republika), Alexandra OLTOVÁ (203 Česká republika), Vlasta LINKOVÁ (203 Česká republika), Petr KUGLÍK (203 Česká republika, garant), Jaroslav ŠTĚRBA (203 Česká republika), Martina PEŠÁKOVÁ (203 Česká republika), Vladimíra VRANOVÁ (703 Slovensko), Denisa MENDELOVÁ (203 Česká republika), Dita ŽEŽULKOVÁ (203 Česká republika) a Hana FILKOVÁ (203 Česká republika)

Vydání

Praha, European Journal of Human Genetics, s. 187-187, 2005

Nakladatel

Nature Publishing Group

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.251

Kód RIV

RIV/00216224:14310/05:00014618

Organizační jednotka

Přírodovědecká fakulta

ISSN

Klíčová slova anglicky

Acute leukemia; chromosome aberrations; cytogenetics; FISH; CGH; HR-CGH

Štítky

acute leukemia, CGH, chromosome aberrations, cytogenetics, FISH, HR-CGH

Změněno: 9. 4. 2010 12:23, prof. MUDr. Jaroslav Štěrba, Ph.D.

Anotace

ORIG CZ

V originále

We report results from the study of 33 children with acute lymphoid (25) and myeloid (8) leukaemia. Karyotyping of bone marrow cells is an important step for the precise diagnosis and for the choice of the adequate treatment in leukaemic patients. Twenty six patients were examined in the time of diagnosis and seven in relapse. The cytogenetic analyses were performed on 24-hours bone marrow cells cultured in RPMI 1640 according to the standard methods. GTG banding technique revealed clonal karyotypic abnormalities in 45% (15) samples. Cytogenetic findings were confirmed by fluorescence in situ hybridization (FISH) method. Comparative genomic hybridization (CGH) was used on bone marrow samples obtained from this patients in time of diagnosis or relapse. CGH showed new DNA copy number changes in 70% (23) patients. For the first time we were tested the modification of CGH. We show examples of submicroscopic chromosomal imbalances being detected with this high resolution CGH technique (HR-CGH). By HR-CGH we detected new chromosomal aberation in more than 75% cases. Here we present comparing among GTG-band karyotyping, CGH and HR-CGH results.

Česky

Práce srovnává výtěžnost detekce specifických chromozomových abnormalit u 33 dětských pacientů s akutní leukémií pomocí techniky G-pruhování, FISH, CGH a HR-CGH.

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

MENTZLOVÁ, Dita, Alexandra OLTOVÁ, Vlasta LINKOVÁ, Petr KUGLÍK, Jaroslav ŠTĚRBA, Martina PEŠÁKOVÁ, Vladimíra VRANOVÁ, Denisa MENDELOVÁ, Dita ŽEŽULKOVÁ a Hana FILKOVÁ. The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH. In European Journal of Human Genetics. Praha: Nature Publishing Group, 2005, s. 187. ISSN 1476-5438.

@inproceedings{618592,
   author = {Mentzlová, Dita and Oltová, Alexandra and Linková, Vlasta and Kuglík, Petr and Štěrba, Jaroslav and Pešáková, Martina and Vranová, Vladimíra and Mendelová, Denisa and Žežulková, Dita and Filková, Hana},
   address = {Praha},
   booktitle = {European Journal of Human Genetics},
   keywords = {Acute leukemia; chromosome aberrations; cytogenetics; FISH; CGH; HR-CGH},
   language = {eng},
   location = {Praha},
   pages = {187-187},
   publisher = {Nature Publishing Group},
   title = {The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH},
   year = {2005}
}

TY  - JOUR
ID  - 618592
AU  - Mentzlová, Dita - Oltová, Alexandra - Linková, Vlasta - Kuglík, Petr - Štěrba, Jaroslav - Pešáková, Martina - Vranová, Vladimíra - Mendelová, Denisa - Žežulková, Dita - Filková, Hana
PY  - 2005
TI  - The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH
PB  - Nature Publishing Group
CY  - Praha
KW  - Acute leukemia
KW  - chromosome aberrations
KW  - cytogenetics
KW  - FISH
KW  - CGH
KW  - HR-CGH
N2  - We report results from the study of 33 children with acute lymphoid (25) and myeloid (8) leukaemia. Karyotyping of bone marrow cells is an important step for the precise diagnosis and for the choice of the adequate treatment in leukaemic patients. Twenty six patients were examined in the time of diagnosis and seven in relapse. The cytogenetic analyses were performed on 24-hours bone marrow cells cultured in RPMI 1640 according to the standard methods. GTG banding technique revealed clonal karyotypic abnormalities in 45% (15) samples. Cytogenetic findings were confirmed by fluorescence in situ hybridization (FISH) method. Comparative genomic hybridization (CGH) was used on bone marrow samples obtained from this patients in time of diagnosis or relapse. CGH showed new DNA copy number changes in 70% (23) patients. For the first time we were tested the modification of CGH. We show examples of submicroscopic chromosomal imbalances being detected with this high resolution CGH technique (HR-CGH). By HR-CGH we detected new chromosomal aberation in more than 75% cases. Here we present comparing among GTG-band karyotyping, CGH and HR-CGH results.
ER  -

MENTZLOVÁ, Dita, Alexandra OLTOVÁ, Vlasta LINKOVÁ, Petr KUGLÍK, Jaroslav ŠTĚRBA, Martina PEŠÁKOVÁ, Vladimíra VRANOVÁ, Denisa MENDELOVÁ, Dita ŽEŽULKOVÁ a Hana FILKOVÁ. The yield of chromosomal aberrations in children with acute leukemia using methods GTG banding, FISH, CGH and HR-CGH. In \textit{European Journal of Human Genetics}. Praha: Nature Publishing Group, 2005, s.~187. ISSN~1476-5438.

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