Elimination  voltammetry  with	linear	scan  as  a  new
detection  method   for   DNA	sensors

J 2005

Elimination voltammetry with linear scan as a new detection method for DNA sensors

TRNKOVÁ, Libuše; František JELEN; Jitka PETRLOVÁ; Vojtěch ADAM; David POTĚŠIL et al.

Základní údaje

Originální název

Elimination voltammetry with linear scan as a new detection method for DNA sensors

Název česky

Eliminační voltametrie s lineárním skenem jako nová detekční metoda pro DNA senzory

Autoři

TRNKOVÁ, Libuše; František JELEN; Jitka PETRLOVÁ; Vojtěch ADAM; David POTĚŠIL a René KIZEK

Vydání

SENSORS, 2005, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.208

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/05:00014629

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000233333900009

Klíčová slova anglicky

oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides

Štítky

DNA biosensor, linear sweep voltammetry, Mercury Electrode, oligodeoxynucleotide, sequence of nucleotides

Změněno: 31. 1. 2006 14:36, prof. RNDr. Libuše Trnková, CSc.

Anotace

ORIG CZ

V originále

The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.

Česky

Tato práce popistuje

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

TRNKOVÁ, Libuše; František JELEN; Jitka PETRLOVÁ; Vojtěch ADAM; David POTĚŠIL a René KIZEK. Elimination voltammetry with linear scan as a new detection method for DNA sensors. SENSORS. 2005, roč. 5, 6-10, s. 448-464. ISSN 1424-8220.

@article{618643,
   author = {Trnková, Libuše and Jelen, František and Petrlová, Jitka and Adam, Vojtěch and Potěšil, David and Kizek, René},
   article_number = {6-10},
   keywords = {oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides},
   language = {eng},
   issn = {1424-8220},
   journal = {SENSORS},
   title = {Elimination voltammetry with linear scan as a new detection method for DNA sensors},
   volume = {5},
   year = {2005}
}

TY  - JOUR
ID  - 618643
AU  - Trnková, Libuše - Jelen, František - Petrlová, Jitka - Adam, Vojtěch - Potěšil, David - Kizek, René
PY  - 2005
TI  - Elimination voltammetry with linear scan as a new detection method for DNA sensors
JF  - SENSORS
VL  - 5
IS  - 6-10
SP  - 448-464
EP  - 448-464
SN  - 14248220
KW  - oligodeoxynucleotide
KW  - DNA biosensor
KW  - linear sweep voltammetry
KW  - elimination voltammetry with linear scan
KW  - reduction of adenine and cytosine
KW  - mercury electrode
KW  - sequence of nucleotides
N2  - The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.
ER  -

TRNKOVÁ, Libuše; František JELEN; Jitka PETRLOVÁ; Vojtěch ADAM; David POTĚŠIL a René KIZEK. Elimination voltammetry with linear scan as a new detection method for DNA sensors. \textit{SENSORS}. 2005, roč.~5, 6-10, s.~448-464. ISSN~1424-8220.

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