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@article{618643,
author = {Trnková, Libuše and Jelen, František and Petrlová, Jitka and Adam, Vojtěch and Potěšil, David and Kizek, René},
article_number = {6-10},
keywords = {oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides},
language = {eng},
issn = {1424-8220},
journal = {SENSORS},
title = {Elimination voltammetry with linear scan as a new detection method for DNA sensors},
volume = {5},
year = {2005}
}
TY - JOUR
ID - 618643
AU - Trnková, Libuše - Jelen, František - Petrlová, Jitka - Adam, Vojtěch - Potěšil, David - Kizek, René
PY - 2005
TI - Elimination voltammetry with linear scan as a new detection method for DNA sensors
JF - SENSORS
VL - 5
IS - 6-10
SP - 448-464
EP - 448-464
SN - 14248220
KW - oligodeoxynucleotide
KW - DNA biosensor
KW - linear sweep voltammetry
KW - elimination voltammetry with linear scan
KW - reduction of adenine and cytosine
KW - mercury electrode
KW - sequence of nucleotides
N2 - The paper describes successful coupling of adsorptive transfer stripping (AdTS) and elimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine ( C) and adenine ( A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers) were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing of the ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through the elimination function conserving the diffusion current component and eliminating kinetic and charging current components. This function, which provides the elimination signal in a peak-counterpeak form, increased the current sensitivity for A and C resolution, and for the recognition of bases sequences in ODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scan rate were found. The combination of EVLS with AdTS procedure can be considered as a new detection method in a DNA sensor.
ER -
TRNKOVÁ, Libuše, František JELEN, Jitka PETRLOVÁ, Vojtěch ADAM, David POTĚŠIL a René KIZEK. Elimination voltammetry with linear scan as a new detection method for DNA sensors. \textit{SENSORS}. 2005, roč.~5, 6-10, s.~448-464. ISSN~1424-8220.
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