2005
Retention Behaviour of Basic Azaarenes in Reversed-phase Liquid Chromatography. The effect of mobile phase pH
KOČÍ, Kamila, Joao DA COSTA PESOA, Radim ŠVÁBENSKÝ a Zdeněk ŠIMEKZákladní údaje
Originální název
Retention Behaviour of Basic Azaarenes in Reversed-phase Liquid Chromatography. The effect of mobile phase pH
Název česky
Retenční chování bazických azaarenů v RP-LC. Vliv pH mobilní fáze
Autoři
KOČÍ, Kamila (203 Česká republika), Joao DA COSTA PESOA (620 Portugalsko), Radim ŠVÁBENSKÝ (203 Česká republika) a Zdeněk ŠIMEK (203 Česká republika, garant)
Vydání
Evora, Portugalsko, Book of Abstracts-4Encoro Nacional de Cromatografia, od s. 35-35, 1 s. 2005
Nakladatel
University of Evora
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10406 Analytical chemistry
Stát vydavatele
Portugalsko
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/05:00014655
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Basic Azaarenes; Reversed-phase Liquid Chromatography
Změněno: 31. 1. 2006 16:44, doc. RNDr. Zdeněk Šimek, CSc.
V originále
Azaarenes are of particular concern due to their carcinogenic and mutagenic properties and numerous sources in the environment. Separation of azaarenes by high performance liguid chromatography is usually done in reversed phase mode using octadecylsilica as the proper stationary phase. Neutral azaarenes can be successfully analysed with high efficiency and reproducibility, but separation of basic ones may be problematic because of specific interactions with column packing. These may be responsible for irreversible adsorption, peak tailing, low column efficiency or irreproducible retention. These effects are mainly supposed to be caused by the interactions of the basic compounds with acidic residual silanols of the silica support. Despite of numerous studies and theories, understanding of azaarenes retention processes in RPLC is still quite incomplete. In the present study we analysed the effect of varying mobile phase pH on the retention behaviour of selected basic azaarenes. The character and complexity of the retention has been investigated in the system with two octadecylsilica stationary phases made by different manufacturers.
Česky
Azaarenes are of particular concern due to their carcinogenic and mutagenic properties and numerous sources in the environment. Separation of azaarenes by high performance liguid chromatography is usually done in reversed phase mode using octadecylsilica as the proper stationary phase. Neutral azaarenes can be successfully analysed with high efficiency and reproducibility, but separation of basic ones may be problematic because of specific interactions with column packing. These may be responsible for irreversible adsorption, peak tailing, low column efficiency or irreproducible retention. These effects are mainly supposed to be caused by the interactions of the basic compounds with acidic residual silanols of the silica support. Despite of numerous studies and theories, understanding of azaarenes retention processes in RPLC is still quite incomplete. In the present study we analysed the effect of varying mobile phase pH on the retention behaviour of selected basic azaarenes. The character and complexity of the retention has been investigated in the system with two octadecylsilica stationary phases made by different manufacturers.
Návaznosti
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