SLUNSK, Zdenka, Iva SLANINOVÁ, Jiří ŠINKORA, Marcela VLKOVÁ and Eva TBORSKÁ. Antiproliferative activities of selected benzo[c]phenanthridine alkaloids. In Biomedical Papers. Olomouc, CR: Palacky University, Olomouc, 2005, p. 57-58. ISBN 1213-8118.
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Basic information
Original name Antiproliferative activities of selected benzo[c]phenanthridine alkaloids.
Name in Czech Antiproliferativní aktivity vybraných benzo[c]phenanthridinových alkaloidů
Authors SLUNSK, Zdenka (203 Czech Republic), Iva SLANINOVÁ (203 Czech Republic, guarantor), Jiří ŠINKORA (203 Czech Republic), Marcela VLKOVÁ (203 Czech Republic) and Eva TBORSKÁ (203 Czech Republic).
Edition Olomouc, CR, Biomedical Papers, p. 57-58, 2 pp. 2005.
Publisher Palacky University, Olomouc
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/05:00013263
Organization unit Faculty of Medicine
ISBN 1213-8118
Keywords in English benzo[c]phenanthridine alkaloid; cytotoxicity; apoptosis; MTT assay
Tags apoptosis, benzo[c]phenanthridine alkaloid, cytotoxicity, MTT assay
Tags International impact
Changed by Changed by: prof. MUDr. Iva Slaninová, Ph.D., učo 2105. Changed: 19/1/2007 09:58.
Abstract
Quaternary benzo[c]phenanthridine alkaloids (QBA) fall into a group of isochinoline alkaloids that have phenylalanin as their precursor. They are isolated from many plant species for instance from Papaveraceae, Fumariaceae and Rutaceae families. Sanguinarin and chelerytrin are the two best known QBA and their biological activities have been widely studied (1,2,3). In addition to them about 10 other QBA differing only by substitution on the aromatic rings have been isolated as a minor components in several species. The informations about biological effects of this minor QBA are rare. In this study the biological effect of sanguinarine (SA), chelerythrine (CHE) and their derinates sanguirubine (SR), chelirubine (CHR) and macarpine (MA) were tested. One normal (skin fibroblasts) and 3 tumour cell lines (HeLa; A 431; HL-60) were used as a model object. Cytotoxicity (IC50) and apoptosis on living cells were studied. After 72 hours, MTT assay was performed to evaluate the in vitro cytotoxicity induced by tested alkaloids at concentration 0,01-5ěg/ml. Promyelocytic leukemia cell line HL-60 has been proved the most sensitive to the alkaloid treatment followed by skin fibroblasts, while the carcinoma cell lines HeLa and A-431 appeared much more resistant. Cytotoxicity of individual alkaloids descended (the value of IC50 increased) in this order CHE > MA > SA > SR > CHR. For apoptosis detection morphology of nuclei of alkaloid treated HL-60 cells after DAPI staining was studied and flow cytometric analysis using Annexin V-FITC and PI was applied. SA, CHE, MA and SR appeared to be inductors of apoptosis. This work was supported by the grants 525/040017 and 301/03/H005 from the Grant agency of Czech republic and MSM0021622415 from Ministry of Education of the Czech Republic
Abstract (in Czech)
Kvartern benzo[c]phenanthridine alkaloids (QBA) pat do skupiny isochinolinovch alkaloid, jejich prekursorem je phenylalanin. Vyskytuj se v rostlinch eled Papaveraceae, Fumariaceae a Rutaceae. Nejznmj a nejlpe prostudovan jsou Sanguinarin and chelerytrin, informace o biologickch aktivitch minoritnch derivt jsou vzcn. Tato studie se zabv srovnnm antiproliferan aktivity sanguinarinu (SA), chelerythrinu (CHE) a minoritnch derivt sanguirubinu (SR), chelirubinu (CHR) a macarpinu (MA) na linii normlnch lidskch konch fibroblast a 3 ndorov bunn linie (HeLa; A 431; HL-60). Antiproliferan aktivita byla stanovena po 72 hodinov kultivaci pomoc MTT assae, schopnost indukovat apoptzu byla studovna pomoc kombinace Anexinu V a propidium jodidu.
Links
GA525/04/0017, research and development projectName: Rostlinné zdroje benzofenanthridinových alkaloidů a účinek těchto alkaloidů na normální a nádorové buňky
Investor: Czech Science Foundation, Plant sources of benzophenanthridine alkaloids and effects of these alkaloid on normal and cancer cells
GD301/03/H005, research and development projectName: Molekulární a buněčná biologie v biomedicíně
Investor: Czech Science Foundation, Molecular and cell biology in biomedicine
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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