Changes of molecular composition in the basal laminae around
the neuron-satellite cell units of the dorsal root ganglia
following constriction nerve injury are related with invasion
of ED-1 positive macrophages

D 2005

Changes of molecular composition in the basal laminae around the neuron-satellite cell units of the dorsal root ganglia following constriction nerve injury are related with invasion of ED-1 positive macrophages

DUBOVÝ, Petr; Radim JANÁLEK a Ilona KLUSÁKOVÁ

Základní údaje

Originální název

Changes of molecular composition in the basal laminae around the neuron-satellite cell units of the dorsal root ganglia following constriction nerve injury are related with invasion of ED-1 positive macrophages

Název česky

Změny se složení lamina basalis kolem strukturálních jednotek neuron-satelitní buňky po poškození periferního nervu

Autoři

DUBOVÝ, Petr; Radim JANÁLEK a Ilona KLUSÁKOVÁ

Vydání

Amsterdam, VII.European Meeting on Glial Cell Function in Health and Disease, s. 112-112, 2005

Nakladatel

VII.European Meeting on Glial Cell Function in Health and Disease

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

neuropathic; pain

Štítky

neuropathic, pain

Příznaky

Mezinárodní význam

Změněno: 8. 2. 2010 17:18, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

A constriction injury (CI) of the peripheral nerve may stimulate invasion of the sympathetic fiber sprouts into the dorsal root ganglia (DRG) to form sympathetic baskets around DRG neurons. Changes of extracellular matrix composition including the basal lamina may result in a condition of DRG stimulating the growth of sympathetic sprouts. The experimental CI was applied ipsilaterally on the spinal nerve at a 4mm distance from DRG as well as on the sciatic nerve at a 40 mm distance from DRG of Wistar rats. Both ipsilateral and contralateral DRG (L4-L5) were removed 1, 2 and 4 weeks after the operation. The DRG (L4-L5) of intact (naive) rats were used as a control. Cryostat sections were simultaneously immunostained for laminin-1, dystroglycan, nidogen, collagen-IV, concanavalin-A, (con-A) and ED-1. Immunofluorescence staining (IF) for laminin-1 around ipsilateral neuron-satellite cell units was a mosaic following CI in contrast to naive and contralateral DRG. The mosaic laminin distribution was confirmed by con-A. Simultaneous staining for nidogen and collagen-IV confirmed changes of molecular composition in the basal laminae around the neuron-satellite cell units after CI. The molecular changes of the basal laminae correlated with invasion of ED-1 positive macrophages and their close relationships with the DRG neuron-satellite cell units. The results suggested that ED-1+ macrophages play a crucial role for the growth of sympathetic sprouts close to the DRG neurons and for development of sympathetically maintained pain. Supported by 309/03/1199 and MSM0021622404.

Česky

Ligatura n. ischiadicus jako model neuropatické bolesti byla použita pro studium změn v okolí těl neuronů spinálních ganglií.

Návaznosti

MSM0021622404, záměr

Název: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Citovat

DUBOVÝ, Petr; Radim JANÁLEK a Ilona KLUSÁKOVÁ. Changes of molecular composition in the basal laminae around the neuron-satellite cell units of the dorsal root ganglia following constriction nerve injury are related with invasion of ED-1 positive macrophages. In VII.European Meeting on Glial Cell Function in Health and Disease. Amsterdam: VII.European Meeting on Glial Cell Function in Health and Disease, 2005, s. 112.

@inproceedings{626305,
   author = {Dubový, Petr and Janálek, Radim and Klusáková, Ilona},
   address = {Amsterdam},
   booktitle = {VII.European Meeting on Glial Cell Function in Health and Disease},
   keywords = {neuropathic; pain},
   language = {eng},
   location = {Amsterdam},
   pages = {112-112},
   publisher = {VII.European Meeting on Glial Cell Function in Health and Disease},
   title = {Changes of molecular composition in the basal laminae around the neuron-satellite cell units of the dorsal root ganglia following constriction nerve injury are related with invasion of ED-1 positive macrophages},
   year = {2005}
}

TY  - CONF
ID  - 626305
AU  - Dubový, Petr - Janálek, Radim - Klusáková, Ilona
PY  - 2005
TI  - Changes of molecular composition in the basal laminae around the neuron-satellite cell units of the dorsal root ganglia following constriction nerve injury are related with invasion of ED-1 positive macrophages
PB  - VII.European Meeting on Glial Cell Function in Health and Disease
CY  - Amsterdam
KW  - neuropathic
KW  - pain
N2  - A constriction injury (CI) of the peripheral nerve may stimulate invasion of the sympathetic fiber sprouts into the dorsal root ganglia (DRG) to form sympathetic baskets around DRG neurons. Changes of extracellular matrix composition including the basal lamina may result in a condition of DRG stimulating the growth of sympathetic sprouts. The experimental CI was applied ipsilaterally on the spinal nerve at a 4mm distance from DRG as well as on the sciatic nerve at a 40 mm distance from DRG of Wistar rats. Both ipsilateral and contralateral DRG (L4-L5) were removed 1, 2 and 4 weeks after the operation. The DRG (L4-L5) of intact (naive) rats were used as a control. Cryostat sections were simultaneously immunostained for laminin-1, dystroglycan, nidogen, collagen-IV, concanavalin-A, (con-A) and ED-1. Immunofluorescence staining (IF) for laminin-1 around ipsilateral neuron-satellite cell units was a mosaic following CI in contrast to naive and contralateral DRG. The mosaic laminin distribution was confirmed by con-A. Simultaneous staining for nidogen and collagen-IV confirmed changes of molecular composition in the basal laminae around the neuron-satellite cell units after CI. The molecular changes of the basal laminae correlated with invasion of ED-1 positive macrophages and their close relationships with the DRG neuron-satellite cell units. The results suggested that ED-1+ macrophages play a crucial role for the growth of sympathetic sprouts close to the DRG neurons and for development of sympathetically maintained pain. Supported by 309/03/1199 and MSM0021622404.
ER  -

DUBOVÝ, Petr; Radim JANÁLEK a Ilona KLUSÁKOVÁ. Changes of molecular composition in the basal laminae around the neuron-satellite cell units of the dorsal root ganglia following constriction nerve injury are related with invasion of ED-1 positive macrophages. In \textit{VII.European Meeting on Glial Cell Function in Health and Disease}. Amsterdam: VII.European Meeting on Glial Cell Function in Health and Disease, 2005, s.~112.

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