CHANGES OF INTRANEURONAL IMMUNOSTAINING FOR CHONDROITIN
SULPHATE PROTEOGLYCANS IN THE BODIES OF PRIMARY AFFERENT
NEURONS FOLLOWING CONSTRICTION NERVE INJURY

D 2005

CHANGES OF INTRANEURONAL IMMUNOSTAINING FOR CHONDROITIN SULPHATE PROTEOGLYCANS IN THE BODIES OF PRIMARY AFFERENT NEURONS FOLLOWING CONSTRICTION NERVE INJURY

DUBOVÝ, Petr a Soňa MARTEČÍKOVÁ

Základní údaje

Originální název

CHANGES OF INTRANEURONAL IMMUNOSTAINING FOR CHONDROITIN SULPHATE PROTEOGLYCANS IN THE BODIES OF PRIMARY AFFERENT NEURONS FOLLOWING CONSTRICTION NERVE INJURY

Název česky

Intraneuronální změny imunodetekce pro chondroitin sulfát proteoglykan v tělech aferentních neuronů po hcronické konstrikci nervu

Autoři

DUBOVÝ, Petr a Soňa MARTEČÍKOVÁ

Vydání

Czech Republic, Czech Society of Histochemistry and Cytochemistry, s. 132-132, 2005

Nakladatel

Czech Society of Histochemistry and Cytochemistry

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

neuropatická bolest - experimentální model - extracelulární matrix

Klíčová slova anglicky

neuropathic pain - experimental model - extracellular matrix

Štítky

ngvfngf

Příznaky

Mezinárodní význam

Změněno: 8. 2. 2010 17:27, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

Chronic constriction injury is one of the most used experimental models for study of neuropathic pain. The sciatic nerve constriction injury is a partial nerve injury model based on both axotomized and spared neurons. The extracellular matrix molecules have a variety of roles in the nervous system. Chondroitin sulphate proteoglycans (CSPGs) are the most abundant type of proteoglycans in the nervous system that act mainly as barrier forming molecules and belong to major inhibitory regulators of axonal regeneration. Monoclonal antibody CS-56 recognizes both the 4- and 6-sulphated forms of CSPG. The aim of the present study was to investigate an immunofluorescence for CS-56 in the dorsal root ganglion (DRG) neurons of intact rats as well as DRG after constriction of sciatic nerve for 14 and 28 days. The immunostained sections were analyzed by an epifluorescence microscope (Leica DMLB) using a software Lucia to measure intensity (brightness) of immunofluorescence and the size of neuronal bodies. Besides immunostaining in the extracellular matrix of DRG, CS-56 immunofluorescence of different intensities was detected in the bodies of DRG neurons of all size-types. The bodies of largest DRG neurons exhibited higher immunofluorescence intensity than was found in the medium- or small-sized neurons. Significantly increased intensity of immunofluorescence was found only in the medium-sized neurons of ipsilateral DRG 28 days after constriction injury when compared with DRG of naive rats or 14 days after operation. No significant difference of immunofluorescence intensity was measured in the contralateral DRG when compared with those of naive rats or operated side. In conclusion, sciatic nerve constriction used as an experimental model of neuropathic pain stimulated increased immunofluorescence for CS-56 only in the medium-sized neuron bodies. The studies of mechanisms related with elevated CS-56 immunofluorescence in the medium-sized neuronal bodies after sciatic nerve constriction are in progress. Supported by grant GAČR 309/03/1199.

Česky

Experimentální model neuropatické bolesti založený na chronické konstrikcí nervu byl použit ke studiu změn v intraneuonální expresi chondroitin sulfát proteoglykanu. Kvantitativní analýzou obrazu bylo zjištěno, že ke zvýšení exprese CSPG dochází pouze v neuronech střední velikosti v ipsilaterálních spinálních gangliích.

Návaznosti

MSM0021622404, záměr

Název: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Citovat

DUBOVÝ, Petr a Soňa MARTEČÍKOVÁ. CHANGES OF INTRANEURONAL IMMUNOSTAINING FOR CHONDROITIN SULPHATE PROTEOGLYCANS IN THE BODIES OF PRIMARY AFFERENT NEURONS FOLLOWING CONSTRICTION NERVE INJURY. In Czech Society of Histochemistry and Cytochemistry. Czech Republic: Czech Society of Histochemistry and Cytochemistry, 2005, s. 132.

@inproceedings{626384,
   author = {Dubový, Petr and Martečíková, Soňa},
   address = {Czech Republic},
   booktitle = {Czech Society of Histochemistry and Cytochemistry},
   keywords = {neuropathic pain - experimental model - extracellular matrix},
   language = {eng},
   location = {Czech Republic},
   pages = {132-132},
   publisher = {Czech Society of Histochemistry and Cytochemistry},
   title = {CHANGES OF INTRANEURONAL IMMUNOSTAINING FOR CHONDROITIN SULPHATE PROTEOGLYCANS IN THE BODIES OF PRIMARY AFFERENT NEURONS FOLLOWING CONSTRICTION NERVE INJURY},
   year = {2005}
}

TY  - CONF
ID  - 626384
AU  - Dubový, Petr - Martečíková, Soňa
PY  - 2005
TI  - CHANGES OF INTRANEURONAL IMMUNOSTAINING FOR CHONDROITIN SULPHATE PROTEOGLYCANS IN THE BODIES OF PRIMARY AFFERENT NEURONS FOLLOWING CONSTRICTION NERVE INJURY
PB  - Czech Society of Histochemistry and Cytochemistry
CY  - Czech Republic
KW  - neuropathic pain - experimental model - extracellular matrix
N2  - Chronic constriction injury is one of the most used experimental models for study of neuropathic pain. The sciatic nerve constriction injury is a partial nerve injury model based on both axotomized and spared neurons. The extracellular matrix molecules have a variety of roles in the nervous system. Chondroitin sulphate proteoglycans (CSPGs) are the most abundant type of proteoglycans in the nervous system that act mainly as barrier forming molecules and belong to major inhibitory regulators of axonal regeneration. Monoclonal antibody CS-56 recognizes both the 4- and 6-sulphated forms of CSPG. The aim of the present study was to investigate an immunofluorescence for CS-56 in the dorsal root ganglion (DRG) neurons of intact rats as well as DRG after constriction of sciatic nerve for 14 and 28 days. The immunostained sections were analyzed by an epifluorescence microscope (Leica DMLB) using a software Lucia to measure intensity (brightness) of immunofluorescence and the size of neuronal bodies. Besides immunostaining in the extracellular matrix of DRG, CS-56 immunofluorescence of different intensities was detected in the bodies of DRG neurons of all size-types. The bodies of largest DRG neurons exhibited higher immunofluorescence intensity than was found in the medium- or small-sized neurons. Significantly increased intensity of immunofluorescence was found only in the medium-sized neurons of ipsilateral DRG 28 days after constriction injury when compared with DRG of naive rats or 14 days after operation. No significant difference of immunofluorescence intensity was measured in the contralateral DRG when compared with those of naive rats or operated side. In conclusion, sciatic nerve constriction used as an experimental model of neuropathic pain stimulated increased immunofluorescence for CS-56 only in the medium-sized neuron bodies. The studies of mechanisms related with elevated CS-56 immunofluorescence in the medium-sized neuronal bodies after sciatic nerve constriction are in progress. Supported by grant GAČR 309/03/1199.
ER  -

DUBOVÝ, Petr a Soňa MARTEČÍKOVÁ. CHANGES OF INTRANEURONAL IMMUNOSTAINING FOR CHONDROITIN SULPHATE PROTEOGLYCANS IN THE BODIES OF PRIMARY AFFERENT NEURONS FOLLOWING CONSTRICTION NERVE INJURY. In \textit{Czech Society of Histochemistry and Cytochemistry}. Czech Republic: Czech Society of Histochemistry and Cytochemistry, 2005, s.~132.

Přehled o publikaci