Instrumentation strategy for investigation of bacteriophage 812
proteome by on-line combination of capillary electrophoresis,
electrochromatography and liquid chromatography with mass
spectrometry

D 2005

Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry

KLEPÁRNÍK, Karel, František FORET, Michal SPĚŠNÝ, Jan PREISLER, Zbyněk ZDRÁHAL et. al.

Základní údaje

Originální název

Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry

Název česky

Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry

Autoři

KLEPÁRNÍK, Karel (203 Česká republika, garant), František FORET (203 Česká republika), Michal SPĚŠNÝ (203 Česká republika), Jan PREISLER (203 Česká republika), Zbyněk ZDRÁHAL (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika)

Vydání

New Orleans, LA USA, 18th International Symposium on MicroScale Bioseparations (MSB), od s. 09-89-Tu, 1 s. 2005

Nakladatel

California Separation Science Society (CaSSS)

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/05:00013274

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

BACTERIOPHAGE; PROTEOME; CAPILLARY ELECTROPHORESIS; ELECTROCHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY

Štítky

Bacteriophage, Capillary electrophoresis, ELECTROCHROMATOGRAPHY, liquid chromatography, mass spectrometry, proteome

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 10. 2008 15:02, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

ORIG CZ

V originále

We have developed a new miniaturized instrumentation for combination of the microscale liquid phase separation techniques with mass spectrometry (MS). Its capability will be demonstrated on analyses of proteins prepared from bacteriophage 812. The optimum conditions for growing bacteriophages, preparation of phage proteins and their tryptic digests, and the capillary separations provided a satisfactory amount of peptides for the protein identification by MS with electrospray. The capillary separation columns and nano-spray needles made of narrow bore (ID 5 50 micrometers) silica-fused capillaries were connected by an original laboratory-made interface. The ultimate goal of our research is to contribute to the understanding of the molecular principle of phage therapy. The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections. The knowledge of bacteriophages lytic enzymes and cell growth inhibitors can potentially lead to the development of low-molecular species with a specific therapeutic activity.

Česky

neuvedeno

Návaznosti

GA203/03/0515, projekt VaV

Název: Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie

Investor: Grantová agentura ČR, Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie

Citovat

KLEPÁRNÍK, Karel, František FORET, Michal SPĚŠNÝ, Jan PREISLER, Zbyněk ZDRÁHAL a Jiří DOŠKAŘ. Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry. In 18th International Symposium on MicroScale Bioseparations (MSB). New Orleans, LA USA: California Separation Science Society (CaSSS), 2005, s. 09-89-Tu, 1 s.

@inproceedings{627884,
   author = {Klepárník, Karel and Foret, František and Spěšný, Michal and Preisler, Jan and Zdráhal, Zbyněk and Doškař, Jiří},
   address = {New Orleans, LA USA},
   booktitle = {18th International Symposium on MicroScale Bioseparations (MSB)},
   keywords = {BACTERIOPHAGE; PROTEOME; CAPILLARY ELECTROPHORESIS; ELECTROCHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY},
   language = {eng},
   location = {New Orleans, LA USA},
   pages = {09-89-Tu},
   publisher = {California Separation Science Society (CaSSS)},
   title = {Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry},
   url = {http://www.casss.org/meetings/2005msb/default.htm},
   year = {2005}
}

TY  - JOUR
ID  - 627884
AU  - Klepárník, Karel - Foret, František - Spěšný, Michal - Preisler, Jan - Zdráhal, Zbyněk - Doškař, Jiří
PY  - 2005
TI  - Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry
PB  - California Separation Science Society (CaSSS)
CY  - New Orleans, LA USA
KW  - BACTERIOPHAGE
KW  - PROTEOME
KW  - CAPILLARY ELECTROPHORESIS
KW  - ELECTROCHROMATOGRAPHY
KW  - LIQUID CHROMATOGRAPHY
KW  - MASS SPECTROMETRY
UR  - http://www.casss.org/meetings/2005msb/default.htm
N2  - We have developed a new miniaturized instrumentation for combination of the microscale liquid phase separation techniques with mass spectrometry (MS). Its capability will be demonstrated on analyses of proteins prepared from bacteriophage 812. The optimum conditions for growing bacteriophages, preparation of phage proteins and their tryptic digests, and the capillary separations provided a satisfactory amount of peptides for the protein identification by MS with electrospray. The capillary separation columns and nano-spray needles made of narrow bore (ID 5 50 micrometers) silica-fused capillaries were connected by an original laboratory-made interface. The ultimate goal of our research is to contribute to the understanding of the molecular principle of phage therapy. The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections. The knowledge of bacteriophages lytic enzymes and cell growth inhibitors can potentially lead to the development of low-molecular species with a specific therapeutic activity.
ER  -

KLEPÁRNÍK, Karel, František FORET, Michal SPĚŠNÝ, Jan PREISLER, Zbyněk ZDRÁHAL a Jiří DOŠKAŘ. Instrumentation strategy for investigation of bacteriophage 812 proteome by on-line combination of capillary electrophoresis, electrochromatography and liquid chromatography with mass spectrometry. In \textit{18th International Symposium on MicroScale Bioseparations (MSB)}. New Orleans, LA USA: California Separation Science Society (CaSSS), 2005, s.~09-89-Tu, 1 s.

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