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@proceedings{630249,
author = {Vařecha, Miroslav and Amrichová, Jana and Ondřej, Vladan and Lukášová, Emilie and Ulman, Vladimír and Matula, Petr and Kozubek, Michal and Kozubek, Stanislav},
booktitle = {Book of Abstracts for 13th Euroconference on Apoptosis},
keywords = {living cells; GFP; fluorescent protein; FRET; fluorescent microscopy},
language = {eng},
isbn = {963-219-273-7},
title = {Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.},
year = {2005}
}
TY - CONF
ID - 630249
AU - Vařecha, Miroslav - Amrichová, Jana - Ondřej, Vladan - Lukášová, Emilie - Ulman, Vladimír - Matula, Petr - Kozubek, Michal - Kozubek, Stanislav
PY - 2005
TI - Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
SN - 9632192737
KW - living cells
KW - GFP
KW - fluorescent protein
KW - FRET
KW - fluorescent microscopy
N2 - Our project is focused on study of mitochondrial apoptogenic proteins released during apoptosis and associated with caspase-independent nuclear chromatin condensation and degradation. We introduce our automated 2D/3D/4D/FRET high-resolution image data acquisition and analysis system developed to visualize and quantify fluorescence signals in fixed and living cells. For our studies, we utilize the fluorescent proteins and various low-toxicity cell permeable fluorescent probes that make it possible to conduct the non-invasive quantitative visualization in living cells. In our experiments, cells are stably or transiently transfected or cotransfected by lipofection with DNA plasmids coding fusion proteins of mitochondrial apoptogenic proteins (cytochrom c, AIF, amid, and endonuclease g) with one of fluorescence proteins (EGFP, EYFP, or t-HcRed).
ER -
VAŘECHA, Miroslav, Jana AMRICHOVÁ, Vladan ONDŘEJ, Emilie LUKÁŠOVÁ, Vladimír ULMAN, Petr MATULA, Michal KOZUBEK a Stanislav KOZUBEK. Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells. In \textit{Book of Abstracts for 13th Euroconference on Apoptosis}. 2005. ISBN~963-219-273-7.
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