| BRABEC, Viktor a Ctirad HOFR. Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II). BIOPOLYMERS. 111 RIVER ST, HOBOKEN, NJ 07030 USA: JOHN WILEY & SONS INC, 2005, roč. 77, č. 4, s. 222-229, 9 s. ISSN 0006-3525. |
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@article{632127,
author = {Brabec, Viktor and Hofr, Ctirad and Hofr, Ctirad},
article_location = {111 RIVER ST, HOBOKEN, NJ 07030 USA},
article_number = {4},
keywords = {DNA; interstrand cross-link; transplatin; calorimetry; van't Hoff enthalpy; circular dichroism},
language = {eng},
issn = {0006-3525},
journal = {BIOPOLYMERS},
title = {Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)},
url = {http://www3.interscience.wiley.com/cgi-bin/fulltext/109873226/PDFSTART},
volume = {77},
year = {2005}
}
TY - JOUR
ID - 632127
AU - Brabec, Viktor - Hofr, Ctirad - Hofr, Ctirad
PY - 2005
TI - Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)
JF - BIOPOLYMERS
VL - 77
IS - 4
SP - 222-229
EP - 222-229
PB - JOHN WILEY & SONS INC
SN - 00063525
KW - DNA
KW - interstrand cross-link
KW - transplatin
KW - calorimetry
KW - van't Hoff enthalpy
KW - circular dichroism
UR - http://www3.interscience.wiley.com/cgi-bin/fulltext/109873226/PDFSTART
N2 - The effect of the location of the interstrand cross-link formed by trans-diamminedichloroplatinum( II) (transplatin) on the thermal stability and energetics of 15-mer DNA duplex has been investigated. The duplex containing single, site-specific cross-link, thermodynamically equivalent model structures (hairpins) and nonmodified duplexes were characterized by differential scanning calorimetry, temperature-dependent uv absorption, and circular dichroism. The results demonstrate that the formation of the interstrand cross-link of transplatin does not affect pronouncedly thermodynamic stability of DNA: the cross-link induces no marked changes not only in enthalpy, but also in reduced (concentration independent) monomolecular transition entropy. These results are consistent with the previous observations that interstrand cross-links of transplatin structurally perturb DNA only to a relatively small extent. On the other hand, constraining the duplex with the interstrand cross-link of transplatin results in a significant increase in thermal stability that is primarily due to entropic effects: the cross-link reduces the molecularity of the oligomer system from bimolecular to monomolecular. Importantly, the position of the interstrand cross-link within the duplex modulates cooperativity of the melting transition of the duplex and consequently its thermal stability.
ER -
BRABEC, Viktor a Ctirad HOFR. Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II). \textit{BIOPOLYMERS}. 111 RIVER ST, HOBOKEN, NJ 07030 USA: JOHN WILEY \&{} SONS INC, 2005, roč.~77, č.~4, s.~222-229, 9 s. ISSN~0006-3525.
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