BENEŠ, Petr, A. VASHISHTA, S. SARASWAT-OHRI, M. FUSEK, Šárka POSPÍŠILOVÁ, Boris TICHÝ and V. VĚTVIČKA. Effect of procathepsin D activation peptide on gene expression of breast cancer cells. Cancer Lett. 2005, vol. 239, No 13, 9 pp. ISSN 0304-3835.
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Basic information
Original name Effect of procathepsin D activation peptide on gene expression of breast cancer cells
Name in Czech Efekt aktivačního peptidu procathepsinu D na genovou expresi buněk karcinomu prsu
Authors BENEŠ, Petr, A. VASHISHTA, S. SARASWAT-OHRI, M. FUSEK, Šárka POSPÍŠILOVÁ, Boris TICHÝ and V. VĚTVIČKA.
Edition Cancer Lett, 2005, 0304-3835.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30200 3.2 Clinical medicine
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.049
Organization unit Faculty of Medicine
UT WoS 000239553500006
Keywords in English breast cancer; gene expression
Tags breast cancer, GENE EXPRESSION
Changed by Changed by: MVDr. Boris Tichý, Ph.D., učo 128327. Changed: 6/4/2010 10:00.
Abstract
Overexpression of procathepsin D (pCD) is reported to occur in numerous types of cancer and is associated with increased growth and metastasis. It has been established that pCD affects multiple stages of tumor progression including proliferation, angiogenesis and metastasis. Previously, we showed that the mitogenic effect of pCD on cancer cells is mediated by interaction of its activation peptide (AP) with yet unidentified cell surface receptor. In this investigation, gene expression profiles were compared between AP-treated and control human breast cancer ZR-75-1 cells to elucidate the mechanism of AP mitogenicity. Several differentially expressed genes involved in signal transduction, regulation of cell cycle, apoptosis, tumor invasion and metastasis were identified using microarray technology. These findings, including overexpression of NF-kappaB2, were confirmed in breast cancer cell lines by reverse-transcriptase PCR (RT-PCR). Understanding the mechanism of pCDs effect on breast cancer cells could extend possibilities of breast cancer treatment in the future.
Abstract (in Czech)
Zvýšená exprese procathepsinu D (pCD) byla popsána u mnoha typů nádorových buněk a byla asociována s vyšší růstovou aktivitou a zvýšenou schopnosí metastazovat. Bylo prokázáno, že pCD ovlivňuje procesy jako je proliferace, angiogeneze a metastazování. Dříve jsme prokázali, že mitogenní efekt pCD je způsoben interakcí jeho aktivačního peptidu (AP) s dosud neznámým povrchovým receptorem. Tyto objevy, zahrnující i zvýšenou expresi NF-kappaB2, byly potvrzeny pomocí RT-PCR. Pochopení efektu pCD u buněk karcinomu prsu může v budoucnosti vést k rozšíření možností protinádorové terapie.
Links
NR8448, research and development projectName: Charakterizace genové exprese leukemických buněk pomocí DNA čipů a její využití pro molekulární diagnostiku a predikci odpovědi na léčbu
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