Significance of qualitative PCR detection metod for preemtive
therapy of cytomegalovirus infection in patiens after
allogeneic hematopoietic stem cell transplantation -
single-centre experience

J 2005

Significance of qualitative PCR detection metod for preemtive therapy of cytomegalovirus infection in patiens after allogeneic hematopoietic stem cell transplantation - single-centre experience

ŠKAPOVÁ, Denisa, Zdeněk RÁČIL, Dana DVOŘÁKOVÁ, Dagmar MINAŘÍKOVÁ, Jiří MAYER et. al.

Základní údaje

Originální název

Significance of qualitative PCR detection metod for preemtive therapy of cytomegalovirus infection in patiens after allogeneic hematopoietic stem cell transplantation - single-centre experience

Autoři

ŠKAPOVÁ, Denisa, Zdeněk RÁČIL, Dana DVOŘÁKOVÁ, Dagmar MINAŘÍKOVÁ a Jiří MAYER

Vydání

Neoplasma, Bratislava, Veda, Slovak Academy Sciences, 2005, 0028-2685

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.731

Organizační jednotka

Lékařská fakulta

UT WoS

000228311600008

Klíčová slova anglicky

cytomegalovirus; polymerase chain reaction; allogeneic hematopoietic stem cell transplantation

Změněno: 3. 11. 2010 15:25, prof. MUDr. Zdeněk Ráčil, Ph.D.

Anotace

V originále

Both early cytomegalovirus (CMV) monitoring and prophylactic antiviral therapy can decrease clinical complications or can prevent them in patients after allogeneic hematopoietic stem cell transplantation (HSCT). Presented paper summarizes experiences with using regular monitoring of reactivation of CMV after allogencic HSCT by qualitative polymerase chain reaction (PCR) method to prevent the development of symptomatic CMV disease. Samples of peripheral blood leukocytes (PBL) in 71 patients were monitored. Because of retransplantations in two patients, 73 transplantations, each followed by the monitoring, were performed. Patients were monitored weekly after the transplantation for CMV DNAemia in PBL. An episode of CMV infection representing an indication for pre-emptive ganciclovir (GCV) or foscarnet (FOS) therapy was defined as two consecutive positive PCR results in 4-7 days. Median time of monitoring was 313 days. The CMV infection was found in 28/73 monitorings (38.4 %) and always was followed by pre-emptive therapy. One recurrence of CMV infection was observed in 4/28 (14.3 %) monitorings and two recurrences in 1/28 (3.6 %) monitorings. Presented approach resulted in complete prevention of overt CMV disease and this study enable to show that qualitative PCR method for determination of incipient CMV infection followed by pre-emptive therapy is suitable for preventing patients after allogeneic transplantation from CMV disease.

Citovat

ŠKAPOVÁ, Denisa, Zdeněk RÁČIL, Dana DVOŘÁKOVÁ, Dagmar MINAŘÍKOVÁ a Jiří MAYER. Significance of qualitative PCR detection metod for preemtive therapy of cytomegalovirus infection in patiens after allogeneic hematopoietic stem cell transplantation - single-centre experience. Neoplasma. Bratislava: Veda, Slovak Academy Sciences, 2005, roč. 52, č. 2, s. 137-142. ISSN 0028-2685.

@article{633789,
   author = {Škapová, Denisa and Ráčil, Zdeněk and Dvořáková, Dana and Minaříková, Dagmar and Mayer, Jiří},
   article_location = {Bratislava},
   article_number = {2},
   keywords = {cytomegalovirus; polymerase chain reaction; allogeneic hematopoietic stem cell transplantation},
   issn = {0028-2685},
   journal = {Neoplasma},
   title = {Significance of qualitative PCR detection metod for preemtive therapy of cytomegalovirus infection in patiens after allogeneic hematopoietic stem cell transplantation - single-centre experience},
   volume = {52},
   year = {2005}
}

TY  - JOUR
ID  - 633789
AU  - Škapová, Denisa - Ráčil, Zdeněk - Dvořáková, Dana - Minaříková, Dagmar - Mayer, Jiří
PY  - 2005
TI  - Significance of qualitative PCR detection metod for preemtive therapy of cytomegalovirus infection in patiens after allogeneic hematopoietic stem cell transplantation - single-centre experience
JF  - Neoplasma
VL  - 52
IS  - 2
SP  - 137-142
EP  - 137-142
PB  - Veda, Slovak Academy Sciences
SN  - 00282685
KW  - cytomegalovirus
KW  - polymerase chain reaction
KW  - allogeneic hematopoietic stem cell transplantation
N2  - Both early cytomegalovirus (CMV) monitoring and prophylactic antiviral therapy can decrease clinical complications or can prevent them in patients after allogeneic hematopoietic stem cell transplantation (HSCT). Presented paper summarizes experiences with using regular monitoring of reactivation of CMV after allogencic HSCT by qualitative polymerase chain reaction (PCR) method to prevent the development of symptomatic CMV disease. Samples of peripheral blood leukocytes (PBL) in 71 patients were monitored. Because of retransplantations in two patients, 73 transplantations, each followed by the monitoring, were performed. Patients were monitored weekly after the transplantation for CMV DNAemia in PBL. An episode of CMV infection representing an indication for pre-emptive ganciclovir (GCV) or foscarnet (FOS) therapy was defined as two consecutive positive PCR results in 4-7 days. Median time of monitoring was 313 days. The CMV infection was found in 28/73 monitorings (38.4 %) and always was followed by pre-emptive therapy. One recurrence of CMV infection was observed in 4/28 (14.3 %) monitorings and two recurrences in 1/28 (3.6 %) monitorings. Presented approach resulted in complete prevention of overt CMV disease and this study enable to show that qualitative PCR method for determination of incipient CMV infection followed by pre-emptive therapy is suitable for preventing patients after allogeneic transplantation from CMV disease.
ER  -

ŠKAPOVÁ, Denisa, Zdeněk RÁČIL, Dana DVOŘÁKOVÁ, Dagmar MINAŘÍKOVÁ a Jiří MAYER. Significance of qualitative PCR detection metod for preemtive therapy of cytomegalovirus infection in patiens after allogeneic hematopoietic stem cell transplantation - single-centre experience. \textit{Neoplasma}. Bratislava: Veda, Slovak Academy Sciences, 2005, roč.~52, č.~2, s.~137-142. ISSN~0028-2685.

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