Electrochemical determination of flavonoids on a carbon paste
electrode in biological samples

D 2006

Electrochemical determination of flavonoids on a carbon paste electrode in biological samples

MIKELOVÁ, Radka; Veronika ŠUPALKOVA; Pavel HANUŠTIAK; Vojtěch ADAM; Petr HODEK et. al.

Základní údaje

Originální název

Electrochemical determination of flavonoids on a carbon paste electrode in biological samples

Název česky

Elektrochemické stanovení flavonoidů na uhlíkov pastové elektrodě v biologických vzorcích

Autoři

MIKELOVÁ, Radka; Veronika ŠUPALKOVA; Pavel HANUŠTIAK; Vojtěch ADAM; Petr HODEK; Marie STIBOROVÁ; Ladislav ZEMAN; Libuše TRNKOVÁ a René KIZEK

Vydání

1. vyd. Bordeaux, 11th International conference on electroanalysis, od s. 44-41, 1 s. 2006

Nakladatel

European Society for ElectroAnalytical Chemistry

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10405 Electrochemistry

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/06:00020010

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Flavonoids; polyphenolic compounds; carbon paste electrode; quercetin; rutin; quercitrin;

Štítky

carbon paste electrode, flavonoids, polyphenolic compounds, quercetin, quercitrin, rutin

Změněno: 20. 6. 2008 13:13, prof. RNDr. Libuše Trnková, CSc.

Anotace

ORIG CZ

V originále

Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge (to form chalcones) or by a pyrane or pyrone ring. Overall, flavonoids have so far been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, hepato- and gastro-protective, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Objective. We suggested electroanalytical technique for determination of quercetin, quercitrin, rutin, diosmin and chrysin on the surface of a carbon paste electrode used for analysis of biological samples (e.g. apples). Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was a carbon paste electrode. Phosphate buffer (0.1 M NaH2PO4 + 0.1 M Na2HPO4, pH 7.0) was used as a supporting electrolyte. Results. Electrochemical procedures represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without disruption of living organism in comparison with the commonly methods used for determination of flavonoids. Here, we suggested the method utilising carbon paste electrode (CPE) for determination of quercetin, rutin, quercitrin, chrysin, and diosmin by square wave voltammetry (SWV) in the presence of phosphate buffer. Polishing using filtration paper prior to this purpose, which is much easier in comparison with glassy carbon electrode, renews the surface of the CPE. Moreover, the influence of glycoside part of the flavonoids on the resulted electrochemical signal was studied. The limits of detection of the flavonoids were about tens of nM, except diosmin (tens of ěM). Finally, we analysed biological samples such as apples. Conclusions. Suggesting of a simple, low cost and sensitive technique for determination of flavonoids in biological material is need due to their benefits to human and animal health. Here, we showed that electrochemical techniques are suitable for these purposes.

Česky

Elektrochemické stanovení flavonoidů na uhlíkové pastové elektrodě v biologických vzorcích

Návaznosti

GP525/04/P132, projekt VaV

Název: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

MIKELOVÁ, Radka; Veronika ŠUPALKOVA; Pavel HANUŠTIAK; Vojtěch ADAM; Petr HODEK; Marie STIBOROVÁ; Ladislav ZEMAN; Libuše TRNKOVÁ a René KIZEK. Electrochemical determination of flavonoids on a carbon paste electrode in biological samples. In 11th International conference on electroanalysis. 1. vyd. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, s. 44-41, 1 s.

@inproceedings{637913,
   author = {Mikelová, Radka and Šupalkova, Veronika and Hanuštiak, Pavel and Adam, Vojtěch and Hodek, Petr and Stiborová, Marie and Zeman, Ladislav and Trnková, Libuše and Kizek, René},
   address = {Bordeaux},
   booktitle = {11th International conference on electroanalysis},
   edition = {1},
   keywords = {Flavonoids; polyphenolic compounds; carbon paste electrode; quercetin; rutin; quercitrin;},
   language = {eng},
   location = {Bordeaux},
   pages = {44-41},
   publisher = {European Society for ElectroAnalytical Chemistry},
   title = {Electrochemical determination of flavonoids on a carbon paste electrode in biological samples},
   year = {2006}
}

TY  - CONF
ID  - 637913
AU  - Mikelová, Radka - Šupalkova, Veronika - Hanuštiak, Pavel - Adam, Vojtěch - Hodek, Petr - Stiborová, Marie - Zeman, Ladislav - Trnková, Libuše - Kizek, René
PY  - 2006
TI  - Electrochemical determination of flavonoids on a carbon paste electrode in biological samples
PB  - European Society for ElectroAnalytical Chemistry
CY  - Bordeaux
KW  - Flavonoids
KW  - polyphenolic compounds
KW  - carbon paste electrode
KW  - quercetin
KW  - rutin
KW  - quercitrin;
N2  - Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge (to form chalcones) or by a pyrane or pyrone ring. Overall, flavonoids have so far been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, hepato- and gastro-protective, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Objective. We suggested electroanalytical technique for determination of quercetin, quercitrin, rutin, diosmin and chrysin on the surface of a carbon paste electrode used for analysis of biological samples (e.g. apples). Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was a carbon paste electrode. Phosphate buffer (0.1 M NaH2PO4 + 0.1 M Na2HPO4, pH 7.0) was used as a supporting electrolyte. Results. Electrochemical procedures represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without disruption of living organism in comparison with the commonly methods used for determination of flavonoids. Here, we suggested the method utilising carbon paste electrode (CPE) for determination of quercetin, rutin, quercitrin, chrysin, and diosmin by square wave voltammetry (SWV) in the presence of phosphate buffer. Polishing using filtration paper prior to this purpose, which is much easier in comparison with glassy carbon electrode, renews the surface of the CPE. Moreover, the influence of glycoside part of the flavonoids on the resulted electrochemical signal was studied. The limits of detection of the flavonoids were about tens of nM, except diosmin (tens of ěM). Finally, we analysed biological samples such as apples. Conclusions. Suggesting of a simple, low cost and sensitive technique for determination of flavonoids in biological material is need due to their benefits to human and animal health. Here, we showed that electrochemical techniques are suitable for these purposes.
ER  -

MIKELOVÁ, Radka; Veronika ŠUPALKOVA; Pavel HANUŠTIAK; Vojtěch ADAM; Petr HODEK; Marie STIBOROVÁ; Ladislav ZEMAN; Libuše TRNKOVÁ a René KIZEK. Electrochemical determination of flavonoids on a carbon paste electrode in biological samples. In \textit{11th International conference on electroanalysis}. 1. vyd. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, s.~44-41, 1 s.

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