Electrochemical determination of flavonoids on a carbon paste
electrode in biological samples

D 2006

Electrochemical determination of flavonoids on a carbon paste electrode in biological samples

MIKELOVÁ, Radka, Veronika ŠUPALKOVA, Pavel HANUŠTIAK, Vojtěch ADAM, Petr HODEK et. al.

Basic information

Original name

Electrochemical determination of flavonoids on a carbon paste electrode in biological samples

Name in Czech

Elektrochemické stanovení flavonoidů na uhlíkov pastové elektrodě v biologických vzorcích

Authors

MIKELOVÁ, Radka (203 Czech Republic), Veronika ŠUPALKOVA (203 Czech Republic), Pavel HANUŠTIAK (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Petr HODEK (203 Czech Republic), Marie STIBOROVÁ (203 Czech Republic), Ladislav ZEMAN (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor) and René KIZEK (203 Czech Republic)

Edition

1. vyd. Bordeaux, 11th International conference on electroanalysis, p. 44-41, 1 pp. 2006

Publisher

European Society for ElectroAnalytical Chemistry

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/06:00020010

Organization unit

Faculty of Science

Keywords in English

Flavonoids; polyphenolic compounds; carbon paste electrode; quercetin; rutin; quercitrin;

Abstract

ORIG CZ

V originále

Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge (to form chalcones) or by a pyrane or pyrone ring. Overall, flavonoids have so far been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, hepato- and gastro-protective, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Objective. We suggested electroanalytical technique for determination of quercetin, quercitrin, rutin, diosmin and chrysin on the surface of a carbon paste electrode used for analysis of biological samples (e.g. apples). Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was a carbon paste electrode. Phosphate buffer (0.1 M NaH2PO4 + 0.1 M Na2HPO4, pH 7.0) was used as a supporting electrolyte. Results. Electrochemical procedures represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without disruption of living organism in comparison with the commonly methods used for determination of flavonoids. Here, we suggested the method utilising carbon paste electrode (CPE) for determination of quercetin, rutin, quercitrin, chrysin, and diosmin by square wave voltammetry (SWV) in the presence of phosphate buffer. Polishing using filtration paper prior to this purpose, which is much easier in comparison with glassy carbon electrode, renews the surface of the CPE. Moreover, the influence of glycoside part of the flavonoids on the resulted electrochemical signal was studied. The limits of detection of the flavonoids were about tens of nM, except diosmin (tens of ěM). Finally, we analysed biological samples such as apples. Conclusions. Suggesting of a simple, low cost and sensitive technique for determination of flavonoids in biological material is need due to their benefits to human and animal health. Here, we showed that electrochemical techniques are suitable for these purposes.

In Czech

Elektrochemické stanovení flavonoidů na uhlíkové pastové elektrodě v biologických vzorcích

Links

GP525/04/P132, research and development project

Name: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

Citovat

MIKELOVÁ, Radka, Veronika ŠUPALKOVA, Pavel HANUŠTIAK, Vojtěch ADAM, Petr HODEK, Marie STIBOROVÁ, Ladislav ZEMAN, Libuše TRNKOVÁ and René KIZEK. Electrochemical determination of flavonoids on a carbon paste electrode in biological samples. In 11th International conference on electroanalysis. 1st ed. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, p. 44-41, 1 pp.

@inproceedings{637913,
   author = {Mikelová, Radka and Šupalkova, Veronika and Hanuštiak, Pavel and Adam, Vojtěch and Hodek, Petr and Stiborová, Marie and Zeman, Ladislav and Trnková, Libuše and Kizek, René},
   address = {Bordeaux},
   booktitle = {11th International conference on electroanalysis},
   edition = {1},
   keywords = {Flavonoids; polyphenolic compounds; carbon paste electrode; quercetin; rutin; quercitrin;},
   language = {eng},
   location = {Bordeaux},
   pages = {44-41},
   publisher = {European Society for ElectroAnalytical Chemistry},
   title = {Electrochemical determination of flavonoids on a carbon paste electrode in biological samples},
   year = {2006}
}

TY  - JOUR
ID  - 637913
AU  - Mikelová, Radka - Šupalkova, Veronika - Hanuštiak, Pavel - Adam, Vojtěch - Hodek, Petr - Stiborová, Marie - Zeman, Ladislav - Trnková, Libuše - Kizek, René
PY  - 2006
TI  - Electrochemical determination of flavonoids on a carbon paste electrode in biological samples
PB  - European Society for ElectroAnalytical Chemistry
CY  - Bordeaux
KW  - Flavonoids
KW  - polyphenolic compounds
KW  - carbon paste electrode
KW  - quercetin
KW  - rutin
KW  - quercitrin;
N2  - Introduction. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. They are characterized by the presence of two benzene rings that are linked by a 3-carbon bridge (to form chalcones) or by a pyrane or pyrone ring. Overall, flavonoids have so far been found to exhibit a wide spectrum of pharmacological properties, including antioxidative, antiallergic, antiinflammatory, antidiabetic, hepato- and gastro-protective, antiviral, and antineoplastic activities. Their anti-cancer effect has been also investigated. Objective. We suggested electroanalytical technique for determination of quercetin, quercitrin, rutin, diosmin and chrysin on the surface of a carbon paste electrode used for analysis of biological samples (e.g. apples). Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was a carbon paste electrode. Phosphate buffer (0.1 M NaH2PO4 + 0.1 M Na2HPO4, pH 7.0) was used as a supporting electrolyte. Results. Electrochemical procedures represent advantageous possibility of miniaturisation and simultaneous analysis in vivo without disruption of living organism in comparison with the commonly methods used for determination of flavonoids. Here, we suggested the method utilising carbon paste electrode (CPE) for determination of quercetin, rutin, quercitrin, chrysin, and diosmin by square wave voltammetry (SWV) in the presence of phosphate buffer. Polishing using filtration paper prior to this purpose, which is much easier in comparison with glassy carbon electrode, renews the surface of the CPE. Moreover, the influence of glycoside part of the flavonoids on the resulted electrochemical signal was studied. The limits of detection of the flavonoids were about tens of nM, except diosmin (tens of ěM). Finally, we analysed biological samples such as apples. Conclusions. Suggesting of a simple, low cost and sensitive technique for determination of flavonoids in biological material is need due to their benefits to human and animal health. Here, we showed that electrochemical techniques are suitable for these purposes.
ER  -

MIKELOVÁ, Radka, Veronika ŠUPALKOVA, Pavel HANUŠTIAK, Vojtěch ADAM, Petr HODEK, Marie STIBOROVÁ, Ladislav ZEMAN, Libuše TRNKOVÁ and René KIZEK. Electrochemical determination of flavonoids on a carbon paste electrode in biological samples. In \textit{11th International conference on electroanalysis}. 1st ed. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, p.~44-41, 1 pp.

Detailed Information on Publication Record