| PETRLOVÁ, Jitka, David POTĚŠIL, Radka MIKELOVÁ, Ondřej BLAŠTÍK, Vojtěch ADAM, Libuše TRNKOVÁ, František JELEN, Richard PRŮŠA, Jiří KUKAČKA and René KIZEK. Attomole voltammetric determination of metallothionein. Electrochimica Acta. 2006, vol. 51, No 24, p. 51125119-51125126. ISSN 0013-4686. |
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@article{638949,
author = {Petrlová, Jitka and Potěšil, David and Mikelová, Radka and Blaštík, Ondřej and Adam, Vojtěch and Trnková, Libuše and Jelen, František and Průša, Richard and Kukačka, Jiří and Kizek, René},
article_number = {24},
keywords = {Metallothionein; Mercury electrode; Differential pulse voltammetry; Cyclic voltammetry; Brdicka reaction; Adsorptive transfer stripping technique;Reducing agent; Tris(2-carboxyethyl)phosphine; Lead poisoning; Human blood serum},
language = {eng},
issn = {0013-4686},
journal = {Electrochimica Acta},
title = {Attomole voltammetric determination of metallothionein},
volume = {51},
year = {2006}
}
TY - JOUR
ID - 638949
AU - Petrlová, Jitka - Potěšil, David - Mikelová, Radka - Blaštík, Ondřej - Adam, Vojtěch - Trnková, Libuše - Jelen, František - Průša, Richard - Kukačka, Jiří - Kizek, René
PY - 2006
TI - Attomole voltammetric determination of metallothionein
JF - Electrochimica Acta
VL - 51
IS - 24
SP - 51125119
EP - 51125119
SN - 00134686
KW - Metallothionein
KW - Mercury electrode
KW - Differential pulse voltammetry
KW - Cyclic voltammetry
KW - Brdicka reaction
KW - Adsorptive transfer stripping technique;Reducing agent
KW - Tris(2-carboxyethyl)phosphine
KW - Lead poisoning
KW - Human blood serum
N2 - Number of authors have concerned with electrochemical analysis of metallothionein. Recently new electroanalytical techniques enabling determination of MT at picomole level has been suggested. The aim of the presented work was to show advantages and disadvantages of the different electrochemical procedures, which are commonly used for the detection of MT(i) cyclic voltammetry, (ii) differential pulse voltammetry, and (iii) Brdicka reaction. Primarily we aimed on improvement of the mentioned techniques. Using of reducing agent (tris(2-carboxyethyl)phosphine) and combination of the mentioned method with adsorptive transfer stripping technique (AdTS) were the main improvements of the voltammetric method. The detection limits of metallothionein measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and DPV Brdicka reaction were 0.5 pmol, 4 fmol and 10 amol, respectively. In addition AdTS DPV Brdicka reactionwas used for the determination of metallothionein in human blood serum of 11-year-old girl, which were lead poisoned.
ER -
PETRLOVÁ, Jitka, David POTĚŠIL, Radka MIKELOVÁ, Ondřej BLAŠTÍK, Vojtěch ADAM, Libuše TRNKOVÁ, František JELEN, Richard PRŮŠA, Jiří KUKAČKA and René KIZEK. Attomole voltammetric determination of metallothionein. \textit{Electrochimica Acta}. 2006, vol.~51, No~24, p.~51125119-51125126. ISSN~0013-4686.
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