2006
Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models
DUBOVÝ, Petr; Radim JANČÁLEK; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ; Kateřina PEJCHALOVÁ et al.Základní údaje
Originální název
Intra- and Extraneuronal Changes of Immunofluorescence Staining for TNF-a and TNFR1 in the Dorsal Root Ganglia of Rat Peripheral Neuropathic Pain Models
Název česky
Intra- a Extraneuronální změny v imunofluorescenci TNF-a a TNFR1 ve spinálních gangliích potkana v modelech periferní neuropatické bolesti
Autoři
Vydání
Cellular and Molecular Neurobiology, Springer Netherlands, 2006, 0272-4340
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Nizozemské království
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.219
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14110/06:00015796
Organizační jednotka
Lékařská fakulta
UT WoS
Klíčová slova anglicky
proinflammatory cytokines; unilateral nerve injury; bilateral reaction
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 26. 6. 2009 10:33, prof. RNDr. Petr Dubový, CSc.
V originále
1. Several lines of evidence suggest that cytokines and their receptors are initiators of changes in the activity of dorsal root ganglia (DRG) neurons, but their cellular distribution is still very limited or controversial. Therefore, the goal of present study was to investigate immunohistochemical distribution of TNF-a and TNF receptor-1 (TNFR1) proteins in the rat DRG following three types of nerve injury. 2. The unilateral sciatic and spinal nerve ligation as well as the sciatic nerve transection were used to induce changes in the distribution of TNF-a and TNFR1 proteins. The TNF-a and TNFR1 immunofluorescence was assessed in the L4-L5 DRG affected by nerve injury for 1 and 2 weeks, and compared with the contralateral ones and those removed from naive or sham-operated rats. A part of the sections was incubated for simultaneous immunostaining for TNF-a and ED-1. The immunofluorescence brightness was measured by image analysis system (LUCIA-G v4.21) to quantify immunostaining for TNF-a and TNFR1 in the naive, ipsi- and contralateral DRG following nerve injury. 3. The ipsilateral L4-L5 DRG and their contralateral counterparts of the rats operated for nerve injury displayed an increased immunofluorescence (IF) for TNF-a and TNFR1 when compared with DRG harvested from naive or sham-operated rats. The TNFa IF was increased bilaterally in the satellite glial cells (SGC) and contralaterally in the neuronal nuclei following sciatic and spinal nerve ligature. The neuronal bodies and their SGC exhibited bilaterally enhanced IF for TNF-a after sciatic nerve transection for 1 and 2 weeks. In addition, the affected DRG were invaded by ED-1 positive macrophages which displayed simultaneously TNFa IF. The ED-1 positive macrophages were frequently located near the neuronal bodies to occupy a position of the satellites. 4. The sciatic and spinal nerve ligature resulted in an increased TNFR1 IF in the neuronal bodies of both ipsi- and contralateral DRG. The sciatic nerve ligature for 1 week induced a rise in TNFR1 IF in the contralateral DRG neurons and their SGC to a higher level than in the ipsilateral ones. In contrast, the sciatic nerve ligature for 2 weeks caused a similar increase of TNFR1 IF in the neurons and their SGC of both ipsi- and contralateral DRG. The spinal nerve ligature or sciatic nerve transection resulted in an increased TNFR1 IF located at the surface of the ipsilateral DRG neurons, but dispersed IF in the contralateral ones. In addition, the SGC of the contralateral in contrast to ipsilateral DRG displayed a higher TNFR1 IF. 5. Our results suggest more sources of TNF-a protein in the ipsilateral and contralateral DRG following unilateral nerve injury including macrophages, SGC and primary sensory neurons. In addition, the SGC and macrophages, which became to be satellites, are well positioned to regulate activity of the DRG neurons by production of TNF-a molecules. Moreover, the different cellular distribution of TNFR1 in the ipsi- and contralateral DRG may reflect different pathways by which TNF-a effect on the primary sensory neurons can be mediated following nerve injury.
Česky
Kvalitativní a kvantitativní imunohistochemická analýza proteinů TNF-a a jeho receptoru TNFR1 v ipsi- a kontralaterálních spinálních gangliích po různém poškození sedacího nervu (modelu neuropatické bolesti).
Návaznosti
| GA309/03/1199, projekt VaV |
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| MSM0021622404, záměr |
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