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@article{694337, author = {Pekárová, B. and Krátká, J. and Slováček, J.}, article_location = {Praha}, article_number = {2}, keywords = {Phytophthora fragariae; polyclonal and monoclonal antibodies; PTA-ELISA; immunoprinting; dot blot; strawberry;}, issn = {1212-2580}, journal = {Ochrana rostlin}, title = {Utilization of immunochemical methods to detect Phytophthora fragariae in strawberry plants.}, volume = {37}, year = {2001} }
TY - JOUR ID - 694337 AU - Pekárová, B. - Krátká, J. - Slováček, J. PY - 2001 TI - Utilization of immunochemical methods to detect Phytophthora fragariae in strawberry plants. JF - Ochrana rostlin VL - 37 IS - 2 SP - 57-65 EP - 57-65 PB - Ústav zemědělských a potravinářských inf SN - 12122580 KW - Phytophthora fragariae KW - polyclonal and monoclonal antibodies KW - PTA-ELISA KW - immunoprinting KW - dot blot KW - strawberry; N2 - Polyclonal antibodies anti-PfP IgG and anti-PfM IgG and monoclonal antibody MAb29were prepared to detect a quarantine pathogen of strawberry, Phytophthora fragariae. Laboratory rabbits and mice were immunized using purified and unpurified protein extracts from the mycelial mass of the pathogen. Except for Pythium ultimum, the antibodies did not cross-react with other pathogenic fungi, such as Botrytis cinerea, Colletotrichum acutatum, Fusarium sp.,Verticillium albo-atrum. PTA-ELISA was used to test the antibodies. P. fragariae was detected in artificially infected strawberries (cultivars Elsanta, Kama, Vanda) by means of PTA-ELISA, immunoprinting and dot blot. Detection of the pathogen was optimal in undamaged roots or roots with necrotic tips only. At a later stage of infection, when whole roots were necrotic, the crown was more suitable for successful detection. To detect P. fragariae at the early stages of infection it is recommended to use at least two of the three mentioned immunotechniques. ER -
PEKÁROVÁ, B., J. KRÁTKÁ a J. SLOVÁČEK. Utilization of immunochemical methods to detect Phytophthora fragariae in strawberry plants. \textit{Ochrana rostlin}. Praha: Ústav zemědělských a potravinářských inf, 2001, roč.~37, č.~2, s.~57-65. ISSN~1212-2580.
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