PETRÁŠ, Petr, Ivana MACHOVÁ, Roman PANTŮČEK, Monika MAREJKOVÁ, Vladislava RŮŽIČKOVÁ and Jiří DOŠKAŘ. Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006. In 12th International Symposium on Staphylococci & Staphylococcal Infections. Abstract Book. Maastricht, the Netherlands: Maastricht Exhibition & Congres Centre. p. 225. 2006.
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Original name Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006
Name in Czech Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006
Authors PETRÁŠ, Petr (203 Czech Republic), Ivana MACHOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, guarantor), Monika MAREJKOVÁ (703 Slovakia), Vladislava RŮŽIČKOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic).
Edition Abstract Book. Maastricht, the Netherlands, 12th International Symposium on Staphylococci & Staphylococcal Infections, p. 225-225, 2006.
Publisher Maastricht Exhibition & Congres Centre
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/06:00017153
Organization unit Faculty of Science
Keywords in English Staphylococcus aureus; medical microbiology; molecular diagnostics; PVL
Tags medical microbiology, Molecular diagnostics, PVL, Staphylococcus aureus
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Roman Pantůček, Ph.D., učo 842. Changed: 31/10/2008 15:02.
Phenotypic and genotypic characteristics of 59 PVL positive strains isolated from clinical specimens in the Czech Republic are described. Methods: The RPLA method was used for detection of superantigens. To confirm toxigenicity PCR screening for the sea-sej, tst, eta and etb genes was carried out. Resistance to 12 antibiotics was tested by the disk diffusion method. Phage typing was performed by the standard method using the Int. Set of Phages. Carriage of the lukS-PV and lukF-PV genes for PVL and mecA gene was tested by a multiplex PCR method. The MRSA PVL+ strains were characterized by SmaI macrorestriction analysis resolved by PFGE, spa typing, SCCmec typing by multiplex PCR, prophage typing and preliminary matching of prophage types with PVL production. Results: The presence of the lukS-PV and lukF-PV genes was detected in 59 (6.6%) of 889 S. aureus strains isolated mainly from lesions, wound infection, skin and soft tissue abscesses. Production of enterotoxins A-D was found in 12 (20%) of the 59 PVL+ strains, two strains also produced TSST-1 and one strain was a producer of exfoliative toxin A. Only six strains were MRSA and were classified into SCCmec type IV, ordinarily found in community acquired MRSA strains. Genotypic properties of PVL+ MRSA strains were correlated with the known European MRSA types. Conclusions: The Czech Ref. Laboratory for Staphylococci has been paying attention to PVL+ S. aureus strains since 2004. By March 31, 2006, we found 59 (7.8%) of 889 isolates, mainly from lesions, to carry genes for PVL. Most (78%) PVL+ strains were phage typeable and were predominantly classified into phage group II (53%). Six MRSA PVL+ isolates carrying SCCmec type IV yielded five distinct and unrelated SmaI PFGE patterns and were of unrelated spa types 003, 008, 186, 311 and also 044 lukS-lukF positive CA-MRSA, widely disseminated in Europe.
Abstract (in Czech)
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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