Další formáty:
BibTeX
LaTeX
RIS
@proceedings{695994,
author = {Havliš, Jan and Šebela, Marek and Štosová, Tatiana and Tarkowski, Petr and Wielsch, Natalie and Thomas, Henrik and Shevchenko, Andrej},
booktitle = {Abstract Book 17th IMSC Prague},
keywords = {trypsin; tryptic digestion; PMF; modified trypsin},
language = {eng},
title = {Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process},
year = {2006}
}
TY - CONF
ID - 695994
AU - Havliš, Jan - Šebela, Marek - Štosová, Tatiana - Tarkowski, Petr - Wielsch, Natalie - Thomas, Henrik - Shevchenko, Andrej
PY - 2006
TI - Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process
KW - trypsin
KW - tryptic digestion
KW - PMF
KW - modified trypsin
N2 - A typical MS-assisted proteomic routine involves protease trypsin to generate specific fragments for further analysis. As the enzyme shows marginal termostability and undegoes intense autolysis, it became important to improve its properties in this regard. Conjugation of trypsin with e.g. oligosaccharides (maltotriose, raffinose, stachyose) is one the possible ways to solve these problems. Another way might be use of proteases of similar specifity from bacteria. Both approches were tested and particular proteases were characterised by means of their biochemical (pI, mol.mass, kinetics) and MS-related properties (cleavage efficiency).
ER -
HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Petr TARKOWSKI, Natalie WIELSCH, Henrik THOMAS a Andrej SHEVCHENKO. Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process. In \textit{Abstract Book 17th IMSC Prague}. 2006.
|
