Changes in the protein profiles and activities of antioxidant
enzymes in fat body of Galleria mellonella in response to boric
acid

D 2006

Changes in the protein profiles and activities of antioxidant enzymes in fat body of Galleria mellonella in response to boric acid

HYRŠL, Pavel; Ender BÜYÜKGÜZEL a Kemal BÜYÜKGÜZEL

Základní údaje

Originální název

Changes in the protein profiles and activities of antioxidant enzymes in fat body of Galleria mellonella in response to boric acid

Název anglicky

Changes in the protein profiles and activities of antioxidant enzymes in fat body of Galleria mellonella in response to boric acid

Autoři

HYRŠL, Pavel; Ender BÜYÜKGÜZEL a Kemal BÜYÜKGÜZEL

Vydání

Supplementary Abstract Book. Izmir, VIIIth European Congress of Entomology, s. 4-4, 2006

Nakladatel

Entomological Society of Turkey

Další údaje

Typ výsledku

Stať ve sborníku

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

Změněno: 30. 11. 2006 15:32, doc. RNDr. Pavel Hyršl, Ph.D.

Anotace

V originále

Alterations in antioxidative response and protein profiles associated with lipid peroxidation level were investigated in fat body of the greater waxmoth, Galleria mellonella (L.) after boric acid (156, 620, 1250 and 2500 ppm) treatment in final instars (VIIth) and pupae. Sodium dodecyl sulphate–polyacrylamide gradient gel electrophoresis (SDS-PAGGE) was used to determine the protein profiles in fat body. Approximately 16-20 protein fractions in the fat body of VIIth instar larvae and pupae were separated and some others were observed in a poor resolution. Protein concentrations in larval fat body was lesser than in pupae. Except apoLp I, similar fractions as in pupal fat body were also obtained in larvae. Storage proteins of molecular weight 72-84 kDa were significantly more abundant in the pupal fat body than in larval fat body. Malondialdehyde (MDA) content was significantly increased in the fat body of larvae and pupae at high concentrations of boric acid. Superoxide dismutase (SOD) activities were significantly increased while catalase (CAT) activities were significantly decreased in larval fat body at all concentrations of boric acid. However, boric acid also resulted in significant increased CAT activities in pupal fat body. Highest concentrations of boric acid resulted in significantly decreased GST activity in larval and pupal fat body. At lowest concentration, boric acid significantly increased the GPX activity in the larval and pupal fat body in comparison to control. There is a significant correlation between SOD activities and MDA content in the larval fat body of the insect. This study suggests that boric acid-induced oxidative stress may be causative factor in weakening of larval and pupal antioxidative defense system and may cause changes in fat body protein profiles. Key words: G. mellonella, boric acid, proteins, antioxidant enzymes, fat body, malondialdehyde

Citovat

HYRŠL, Pavel; Ender BÜYÜKGÜZEL a Kemal BÜYÜKGÜZEL. Changes in the protein profiles and activities of antioxidant enzymes in fat body of Galleria mellonella in response to boric acid. In VIIIth European Congress of Entomology. Supplementary Abstract Book. Izmir: Entomological Society of Turkey, 2006, s. 4.

@inproceedings{696611,
   author = {Hyršl, Pavel and Büyükgüzel, Ender and Büyükgüzel, Kemal},
   address = {Izmir},
   booktitle = {VIIIth European Congress of Entomology},
   edition = {Supplementary Abstract Book},
   location = {Izmir},
   pages = {4-4},
   publisher = {Entomological Society of Turkey},
   title = {Changes in the protein profiles and activities of antioxidant enzymes in fat body of Galleria mellonella in response to boric acid},
   year = {2006}
}

TY  - CONF
ID  - 696611
AU  - Hyršl, Pavel - Büyükgüzel, Ender - Büyükgüzel, Kemal
PY  - 2006
TI  - Changes in the protein profiles and activities of antioxidant enzymes in fat body of Galleria mellonella in response to boric acid
PB  - Entomological Society of Turkey
CY  - Izmir
N2  - Alterations in antioxidative response and protein profiles associated with lipid peroxidation level were investigated in fat body of the greater waxmoth, Galleria mellonella (L.) after boric acid (156, 620, 1250 and 2500 ppm) treatment in final instars (VIIth) and pupae. Sodium dodecyl sulphate–polyacrylamide gradient gel electrophoresis (SDS-PAGGE) was used to determine the protein profiles in fat body. Approximately 16-20 protein fractions in the fat body of VIIth instar larvae and pupae were separated and some others were observed in a poor resolution. Protein concentrations in larval fat body was lesser than in pupae. Except apoLp I, similar fractions as in pupal fat body were also obtained in larvae. Storage proteins of molecular weight 72-84 kDa were significantly more abundant in the pupal fat body than in larval fat body. Malondialdehyde (MDA) content was significantly increased in the fat body of larvae and pupae at high concentrations of boric acid. Superoxide dismutase (SOD) activities were significantly increased while catalase (CAT) activities were significantly decreased in larval fat body at all concentrations of boric acid. However, boric acid also resulted in significant increased CAT activities in pupal fat body. Highest concentrations of boric acid resulted in significantly decreased GST activity in larval and pupal fat body. At lowest concentration, boric acid significantly increased the GPX activity in the larval and pupal fat body in comparison to control. There is a significant correlation between SOD activities and MDA content in the larval fat body of the insect. This study suggests that boric acid-induced oxidative stress may be causative factor in weakening of larval and pupal antioxidative defense system and may cause changes in fat body protein profiles. Key words: G. mellonella, boric acid, proteins, antioxidant enzymes, fat body, malondialdehyde
ER  -

HYRŠL, Pavel; Ender BÜYÜKGÜZEL a Kemal BÜYÜKGÜZEL. Changes in the protein profiles and activities of antioxidant enzymes in fat body of Galleria mellonella in response to boric acid. In \textit{VIIIth European Congress of Entomology}. Supplementary Abstract Book. Izmir: Entomological Society of Turkey, 2006, s.~4.

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