Adhesion of eukaryotic cell lines on the gold surface modified
with extracellular matrix proteins monitored by the
piezoelectric sensor

J 2007

Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor

FOHLEROVÁ, Zdenka; Petr SKLÁDAL a Jaroslav TURÁNEK

Základní údaje

Originální název

Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor

Název česky

Adheze eukaryotickych buecnych linii na povrch zlata modifikovany extracelularnimi bunecnymi proteiny

Autoři

FOHLEROVÁ, Zdenka; Petr SKLÁDAL a Jaroslav TURÁNEK

Vydání

Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2007, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.061

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/07:00021741

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000245541600009

Klíčová slova anglicky

Piezoelectric biosensor; Reisstance; Resonant frequency; Laminin; Fibronectin; Vitronectin

Štítky

fibronectin, laminin, Piezoelectric biosensor, Reisstance, Resonant frequency, Vitronectin

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 7. 2007 10:29, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

The piezoelectric sensor (quartz crystal microbalance, QCM) was used to monitor cell adhesion in real time. Two cell lines, rat epithelial cells (WB F344) and lung melanoma cells (B16F10) were used. The cells were adhered and grown on the gold surface of the sensor pre-coated with adsorbed layer of extracellular matrix proteins as vitronectin and laminin. The process of cell attachment and spreading on the gold surface was continuously monitored and displayed by changes of the resonant frequency and resistance values of the piezoelectric resonators. The initial phase of cell attachment and spreading induced a decrease of frequency and increase of resistance relating viscoelastic properties of the cell monolayer on the sensing surface. The steady-state of both shifts was achieved after a few hours. The presence and state of cells on the surface was confirmed by fluorescent microscopy. The obtained results demonstrate that the piezoelectric sensor is suitable for studies of the cell adhesion processes. Thus obtained cell-based biosensor has potential for identification and screening of biologically active drugs and other biomolecules affecting cellular shape and attachment.

Česky

Pomoci piezoelektrickeho biosensoru byla studovana adheze bunecnych linii na povrch zlata modifikovany vhodnymi proteiny.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

FOHLEROVÁ, Zdenka; Petr SKLÁDAL a Jaroslav TURÁNEK. Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 2007, roč. 22, 9-10, s. 1896-1901. ISSN 0956-5663.

@article{701750,
   author = {Fohlerová, Zdenka and Skládal, Petr and Turánek, Jaroslav},
   article_location = {Oxford},
   article_number = {9-10},
   keywords = {Piezoelectric biosensor; Reisstance; Resonant frequency; Laminin; Fibronectin; Vitronectin},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors & Bioelectronics},
   title = {Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor},
   url = {http://www.sciencedirect.com/dx.doi.org/10.1016/j.bios.2006.08.015},
   volume = {22},
   year = {2007}
}

TY  - JOUR
ID  - 701750
AU  - Fohlerová, Zdenka - Skládal, Petr - Turánek, Jaroslav
PY  - 2007
TI  - Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor
JF  - Biosensors & Bioelectronics
VL  - 22
IS  - 9-10
SP  - 1896-1901
EP  - 1896-1901
PB  - Elsevier Advanced Technology
SN  - 09565663
KW  - Piezoelectric biosensor
KW  - Reisstance
KW  - Resonant frequency
KW  - Laminin
KW  - Fibronectin
KW  - Vitronectin
UR  - http://www.sciencedirect.com/dx.doi.org/10.1016/j.bios.2006.08.015
N2  - The piezoelectric sensor (quartz crystal microbalance, QCM) was used to monitor cell adhesion in real time. Two cell lines, rat epithelial cells (WB F344) and lung melanoma cells (B16F10) were used. The cells were adhered and grown on the gold surface of the sensor pre-coated with adsorbed layer of extracellular matrix proteins as vitronectin and laminin. The process of cell attachment and spreading on the gold surface was continuously monitored and displayed by changes of the resonant frequency and resistance values of the piezoelectric resonators. The initial phase of cell attachment and spreading induced a decrease of frequency and increase of resistance relating viscoelastic properties of the cell monolayer on the sensing surface. The steady-state of both shifts was achieved after a few hours. The presence and state of cells on the surface was confirmed by fluorescent microscopy. The obtained results demonstrate that the piezoelectric sensor is suitable for studies of the cell adhesion processes. Thus obtained cell-based biosensor has potential for identification and screening of biologically active drugs and other biomolecules affecting cellular shape and attachment.
ER  -

FOHLEROVÁ, Zdenka; Petr SKLÁDAL a Jaroslav TURÁNEK. Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor. \textit{Biosensors \&{} Bioelectronics}. Oxford: Elsevier Advanced Technology, 2007, roč.~22, 9-10, s.~1896-1901. ISSN~0956-5663.

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