| PILNÝ, Radomír, Pavel BOUCHAL, Šárka BOŘILOVÁ, Pavla ČEŠKOVÁ, Jan ŽALOUDÍK, Rostislav VYZULA, Bořivoj VOJTĚŠEK a Dalibor VALÍK. Surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry reveals significant artifacts in serum obtained from clot activator-containing collection devices. Clinical Chemistry. Washington, USA: Amer. Assoc. Clinical Chemistry, 2006, roč. 52, č. 11, s. 2015-2016. ISSN 0009-9147. |
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@article{702484,
author = {Pilný, Radomír and Bouchal, Pavel and Bořilová, Šárka and Češková, Pavla and Žaloudík, Jan and Vyzula, Rostislav and Vojtěšek, Bořivoj and Valík, Dalibor},
article_location = {Washington, USA},
article_number = {11},
keywords = {proteomics; SELDI; MALDI; TOF; preanalytical variables; separator gel; clotting activator; blood collection},
language = {eng},
issn = {0009-9147},
journal = {Clinical Chemistry},
title = {Surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry reveals significant artifacts in serum obtained from clot activator-containing collection devices},
url = {http://www.clinchem.org/cgi/content/full/52/11/2115},
volume = {52},
year = {2006}
}
TY - JOUR
ID - 702484
AU - Pilný, Radomír - Bouchal, Pavel - Bořilová, Šárka - Češková, Pavla - Žaloudík, Jan - Vyzula, Rostislav - Vojtěšek, Bořivoj - Valík, Dalibor
PY - 2006
TI - Surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry reveals significant artifacts in serum obtained from clot activator-containing collection devices
JF - Clinical Chemistry
VL - 52
IS - 11
SP - 2015-2016
EP - 2015-2016
PB - Amer. Assoc. Clinical Chemistry
SN - 00099147
KW - proteomics
KW - SELDI
KW - MALDI
KW - TOF
KW - preanalytical variables
KW - separator gel
KW - clotting activator
KW - blood collection
UR - http://www.clinchem.org/cgi/content/full/52/11/2115
N2 - Mass spectrometric protein profiling and pattern analysis approaches provide exciting opportunities in disease diagnosis and monitoring. Rapid advent of mass spectrometric protein profiling in recent years utilized mostly MALDI/TOF and/or SELDI/TOF platforms to analyze readily available biological materials such as serum or plasma. In our work, we studied changes in protein profiles of specimens taken in routinely used blood collecting media to see whether the newly introduced clot activator and gel-containing tubes may distort the mass spectrometric protein spectra when compared to plain sample collection tubes used previously. Here, we present an extension of previous observation and delineate a blood processing algorithm that will be applicable for analysis and banking of specimens for MALDI/SELDI-based studies in cancer patients to assess only the changes being as closely as possible of biological origin. We evaluated specific differences between groups of collection tubes Microvette Sarstedt type Neutral defined as “white” containing no gel and/or clot activator versus Microvette Sarstedt Serum, Gel Clotting activator denoted “brown” containing both. Altogether, 34 peak clusters were generated from within the mass range analyzed (3000 – 20000 m/z). In the “brown” group we detected two peaks with m/z 3957.3 and 4283.6 displaying grossly increased peak intensities whose average normalized peak areas were more than 40-fold higher comparing with the “white” groups. Remaining peaks did not display any statistical significance with respect to their occurrence in white and/or brown collection tubes, respectively.
ER -
PILNÝ, Radomír, Pavel BOUCHAL, Šárka BOŘILOVÁ, Pavla ČEŠKOVÁ, Jan ŽALOUDÍK, Rostislav VYZULA, Bořivoj VOJTĚŠEK a Dalibor VALÍK. Surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry reveals significant artifacts in serum obtained from clot activator-containing collection devices. \textit{Clinical Chemistry}. Washington, USA: Amer. Assoc. Clinical Chemistry, 2006, roč.~52, č.~11, s.~2015-2016. ISSN~0009-9147.
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