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@proceedings{704273,
author = {Matějková, Petra and Šmajs, David},
booktitle = {International Medical Postgraduate Conference. Abstract Book.},
keywords = {comparative genome sequencing; T. pallidum ssp. pallidum Samoa D},
language = {eng},
title = {Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D.},
year = {2006}
}
TY - CONF
ID - 704273
AU - Matějková, Petra - Šmajs, David
PY - 2006
TI - Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D.
KW - comparative genome sequencing
KW - T. pallidum ssp. pallidum Samoa D
N2 - Spirochetal genus Treponema includes several closely related pathogenic spirochetes. Treponema pallidum ssp. pallidum (TPA) is the causative agent of syphilis, T. pallidum ssp. pertenue (TPE) causes yaws. Although these two subspecies are microscopically and serologically indistinguishable, they cause different diseases. Little is known about genome differences between TPA and TPE genomes. The aim of this work was to employ dideoxyterminator sequencing for sequence differences discovery between Nichols (TPA) and Samoa D (TPE) genomes. All heterologous regions were successfully subjected to DDT sequencing and consensus sequences were obtained using three different approaches: 1) direct PCR product sequencing, 2) primer walking and 3) small insert library sequencing. Identified sequence differences will be used together with CGS data to compile the complete genome sequence of Samoa D strain.
ER -
MATĚJKOVÁ, Petra a David ŠMAJS. Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D. In \textit{International Medical Postgraduate Conference. Abstract Book.}. 2006.
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