WOZNICOVÁ, Vladana, Miroslav VOTAVA and Magdalena FLASAROVÁ. Klinický materiál pro průkaz syfilis metodou polymerázové řetězové reakce. (Clinical specimens for PCR detection of syphilis). Epidemiologie, mikrobiologie, imunologie. Praha: Česká lékařská společnost J. E. Purkyně, 2007, vol. 56, No 2, 5 pp. ISSN 1210-7913.
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Basic information
Original name Klinický materiál pro průkaz syfilis metodou polymerázové řetězové reakce.
Name in Czech Klinický materiál pro průkaz syfilis metodou polymerázové řetězové reakce.
Name (in English) Clinical specimens for PCR detection of syphilis
Authors WOZNICOVÁ, Vladana (203 Czech Republic, guarantor), Miroslav VOTAVA (203 Czech Republic) and Magdalena FLASAROVÁ (203 Czech Republic).
Edition Epidemiologie, mikrobiologie, imunologie. Praha, Česká lékařská společnost J. E. Purkyně, 2007, 1210-7913.
Other information
Original language Czech
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/07:00028185
Organization unit Faculty of Medicine
Keywords in English syphilis; PCR; clinical samples
Tags clinical samples, PCR, syphilis
Changed by Changed by: doc. MUDr. Vladana Woznicová, Ph.D., učo 606. Changed: 31/3/2010 12:16.
Abstract
A review is presented of types of clinical specimens used for diagnosis of syphilis by polymerase chain reaction. PCR is a routine method for detection of T. pallidum in swabs of chancres and primary and secondary syphilis mucocutaneous lesions. Whole blood, cerebrospinal fluid, amniotic fluid, aspirate and biopsy specimens, and paraffin-embedded tissues can also be tested by PCR for T. pallidum. However, further research on PCR detection sensitivity at various stages of syphilis is needed before these specimens are used in clinical practice.
Abstract (in English)
A review is presented of types of clinical specimens used for diagnosis of syphilis by polymerase chain reaction. PCR is a routine method for detection of T. pallidum in swabs of chancres and primary and secondary syphilis mucocutaneous lesions. Whole blood, cerebrospinal fluid, amniotic fluid, aspirate and biopsy specimens, and paraffin-embedded tissues can also be tested by PCR for T. pallidum. However, further research on PCR detection sensitivity at various stages of syphilis is needed before these specimens are used in clinical practice.
Links
NR8967, research and development projectName: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů
Investor: Ministry of Health of the CR
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