Detection of chromosomal aberrations by FISH, CGH and SKY in
cultured and noncultured tumours in children during 2003 and
2005

D 2006

Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005

OLTOVÁ, Alexandra, Petra ŠMUHAŘOVÁ, Petr KUGLÍK, Dita ŽEŽULKOVÁ, Vladimíra VRANOVÁ et. al.

Základní údaje

Originální název

Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005

Název česky

Detekce chromozomových aberací u kultivovaných a nekultivovaných dětských solidních nádorů pomocí techniky FISH, CGH a SKY v průběhu let 2003 až 2005

Autoři

OLTOVÁ, Alexandra (203 Česká republika), Petra ŠMUHAŘOVÁ (203 Česká republika), Petr KUGLÍK (203 Česká republika, garant), Dita ŽEŽULKOVÁ (203 Česká republika), Vladimíra VRANOVÁ (703 Slovensko), Peter MÚDRY (203 Česká republika), Věra BAIČIOVÁ (203 Česká republika) a Jaroslav ŠTĚRBA (203 Česká republika)

Vydání

Amsterdam, Europen Journal of Human Genetics, s. 226-226, 2006

Nakladatel

ESHG

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/06:00017836

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-7232-264-8

Klíčová slova anglicky

Chromosomal aberrations; solid tumours in children; FISH; CGH; SKY

Štítky

CGH, chromosomal aberrations, FISH, SKY, solid tumours in children

Změněno: 23. 3. 2010 12:28, prof. MUDr. Jaroslav Štěrba, Ph.D.

Anotace

ORIG CZ

V originále

Pediatric tumors are the second main cause of childrens death in developed countries. Contrary to leukemias chromosomal alterations associated with malignant transformations are less well characterized for pediatric solid tumors. We present results of cytogenetic examinations of 262 pediatric solid tumors. Some of them were examined using by interphase fluorescent in situ hybridization (I-FISH), especially tumors where deletions or amplifications of specific genes and/or presence of fusion genes are known and could be used as diagnostic tool. Proven prognostic significance of the n-myc amplification, del 1p36, gain 17q, abnormalities on chromosome 11 are well known for children with neuroblastoma. For medulloblastomas i(17p), c-myc and i(12p) in germinal tumors are the other candidates. Vast majority of tumor samples were cultured and other possible chromosomal abnormalities were detected using FISH, spectral karyotyping (SKY) and comparative genomic hybridization (CGH). During last few months we have applied also high-resolution comparative genomic hybridization (HR-CGH). Cultivation of solid tumor tissue is not always successful, possible explanation could be generally low proliferation activity of tumor cells in vitro. Here we present our cytogenetic results together with clinical characteristics.

Česky

Práce se zabývá detekcí chromozomových aberací ve vzorcích kultivované a nekultivované tkáně solidních dětských nádorů pomocí techniky FISH, CGH a spektrálního karyotypování (SKY) Jsou zhodnoceny cytogenetické nálezy u pacientů léčených na KDO FN Brno v letech 2003-2005.

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

OLTOVÁ, Alexandra, Petra ŠMUHAŘOVÁ, Petr KUGLÍK, Dita ŽEŽULKOVÁ, Vladimíra VRANOVÁ, Peter MÚDRY, Věra BAIČIOVÁ a Jaroslav ŠTĚRBA. Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005. In Europen Journal of Human Genetics. Amsterdam: ESHG, 2006, s. 226. ISBN 80-7232-264-8.

@inproceedings{705152,
   author = {Oltová, Alexandra and Šmuhařová, Petra and Kuglík, Petr and Žežulková, Dita and Vranová, Vladimíra and Múdry, Peter and Baičiová, Věra and Štěrba, Jaroslav},
   address = {Amsterdam},
   booktitle = {Europen Journal of Human Genetics},
   keywords = {Chromosomal aberrations; solid tumours in children; FISH; CGH; SKY},
   language = {eng},
   location = {Amsterdam},
   isbn = {80-7232-264-8},
   pages = {226-226},
   publisher = {ESHG},
   title = {Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005},
   year = {2006}
}

TY  - JOUR
ID  - 705152
AU  - Oltová, Alexandra - Šmuhařová, Petra - Kuglík, Petr - Žežulková, Dita - Vranová, Vladimíra - Múdry, Peter - Baičiová, Věra - Štěrba, Jaroslav
PY  - 2006
TI  - Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005
PB  - ESHG
CY  - Amsterdam
SN  - 8072322648
KW  - Chromosomal aberrations
KW  - solid tumours in children
KW  - FISH
KW  - CGH
KW  - SKY
N2  - Pediatric tumors are the second main cause of childrens death in developed countries. Contrary to leukemias chromosomal alterations associated with malignant transformations are less well characterized for pediatric solid tumors. We present results of cytogenetic examinations of 262 pediatric solid tumors. Some of them were examined using by interphase fluorescent in situ hybridization (I-FISH), especially tumors where deletions or amplifications of specific genes and/or presence of fusion genes are known and could be used as diagnostic tool. Proven prognostic significance of the n-myc amplification, del 1p36, gain 17q, abnormalities on chromosome 11 are well known for children with neuroblastoma. For medulloblastomas i(17p), c-myc and i(12p) in germinal tumors are the other candidates. Vast majority of tumor samples were cultured and other possible chromosomal abnormalities were detected using FISH, spectral karyotyping (SKY) and comparative genomic hybridization (CGH). During last few months we have applied also high-resolution comparative genomic hybridization (HR-CGH). Cultivation of solid tumor tissue is not always successful, possible explanation could be generally low proliferation activity of tumor cells in vitro. Here we present our cytogenetic results together with clinical characteristics.
ER  -

OLTOVÁ, Alexandra, Petra ŠMUHAŘOVÁ, Petr KUGLÍK, Dita ŽEŽULKOVÁ, Vladimíra VRANOVÁ, Peter MÚDRY, Věra BAIČIOVÁ a Jaroslav ŠTĚRBA. Detection of chromosomal aberrations by FISH, CGH and SKY in cultured and noncultured tumours in children during 2003 and 2005. In \textit{Europen Journal of Human Genetics}. Amsterdam: ESHG, 2006, s.~226. ISBN~80-7232-264-8.

Podrobný výpis o publikaci