Fish mapping of ribosomal DNA and telomeric repeats in model
species of Platyhelmithes and Acanthocephala

D 2006

Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala

BOMBAROVÁ, Marta, Magda VÍTKOVÁ, František MAREC, Martina PEČÍNKOVÁ, Marta ŠPAKULOVÁ et. al.

Základní údaje

Originální název

Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala

Název česky

Cytogenetické mapování pomocí FISH ribozomální DNA a repetetivních telomerických sekvencí modelových parazitických druhů kmene Plathelminthes a Acanthocephala

Autoři

BOMBAROVÁ, Marta (703 Slovensko), Magda VÍTKOVÁ (203 Česká republika), František MAREC (203 Česká republika), Martina PEČÍNKOVÁ (203 Česká republika, garant), Marta ŠPAKULOVÁ (703 Slovensko) a Ivica HROMADOVÁ (703 Slovensko)

Vydání

Glasgow, Scotland, 11th International Congress of Parasitology, CD-ROM, od s. CD-ROM, 1 s. 2006

Nakladatel

ICOPA XI Congress Secretariat

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

FISH;Platyhelmithes;Acanthocephala

Štítky

Acanthocephala, FISH, Platyhelmithes

Změněno: 18. 12. 2006 12:17, Mgr. Martina Pečínková, Ph.D.

Anotace

ORIG CZ

V originále

Molecular cytogenetics is little exploited in metazoan parasites despite considerable relevance of the karyotype structure and chromosomal location of specific DNA sequences to phylogenetic and systematic evaluations. We applied fluorescence in situ hybridization (FISH) to determine the type of telomeric DNA and localize rDNA genes in chromosomes of several acantocephalan, monogenean and cestode species. In Paradiplozoon homoion-complex (Monogenea, 2n = 12), the chromosome pair no. 5 carried a nucleolar organizer region (NOR) composed of a cluster of rDNA genes as confirmed by both the classical banding approaches and FISH with 18S rDNA probe. In two acanthocephalan sibling species Pomphorhynchus laevis and P. tereticollis (Acanthocephala, both 2n = 6 + XX in female and X0 in male), two NORs were found, located each in different sites of the first two autosomal pairs. Furthermore, we hybridized the "vertebrate" telomeric probe, (TTAGGG)n, with chromosomes of above-mentioned helminths and cestodes Caryophyllaeus laticeps(2n = 20), Caryophyllaeides fennica (2n = 20), and Nippotaenia mogurndae (2n = 28). The probe hybridized with chromosome ends in all Platyhelminthes (Monogenea, Cestoda) but not in Acanthocephala. No hybridization signals in P. laevis and P. tereticollis were also obtained with the "arthropod" (TTAGG)n or "nematode"(TTAGGC)n telomeric probes. Our results suggest that whereas the telomeres in Platyhelminthes are composed of the TTAGGG repeates, this ancestral motif of telomeres in Bilateria was lost in Acanthocephala and replaced with unknown telomeric sequences.

Česky

Byla aplikována fluorescence in situ hybridizace (FISH) pro stanovení typu telomerické DNA a lokalizace rDNA genů chromozomů několika druhů z taxonů Acanthocephala, Monogenea a Cestoda.

Návaznosti

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

BOMBAROVÁ, Marta, Magda VÍTKOVÁ, František MAREC, Martina PEČÍNKOVÁ, Marta ŠPAKULOVÁ a Ivica HROMADOVÁ. Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala. In 11th International Congress of Parasitology, CD-ROM. Glasgow, Scotland: ICOPA XI Congress Secretariat, 2006, s. CD-ROM, 1 s.

@inproceedings{706103,
   author = {Bombarová, Marta and Vítková, Magda and Marec, František and Pečínková, Martina and Špakulová, Marta and Hromadová, Ivica},
   address = {Glasgow, Scotland},
   booktitle = {11th International Congress of Parasitology, CD-ROM},
   keywords = {FISH;Platyhelmithes;Acanthocephala},
   language = {eng},
   location = {Glasgow, Scotland},
   pages = {CD-ROM},
   publisher = {ICOPA XI Congress Secretariat},
   title = {Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala},
   year = {2006}
}

TY  - JOUR
ID  - 706103
AU  - Bombarová, Marta - Vítková, Magda - Marec, František - Pečínková, Martina - Špakulová, Marta - Hromadová, Ivica
PY  - 2006
TI  - Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala
PB  - ICOPA XI Congress Secretariat
CY  - Glasgow, Scotland
KW  - FISH;Platyhelmithes;Acanthocephala
N2  - Molecular cytogenetics is little exploited in metazoan parasites despite considerable relevance of the karyotype structure and chromosomal location of specific DNA sequences to phylogenetic and systematic evaluations. We applied fluorescence in situ hybridization (FISH) to determine the type of telomeric DNA and localize rDNA genes in chromosomes of several acantocephalan, monogenean and cestode species. In Paradiplozoon homoion-complex (Monogenea, 2n = 12), the chromosome pair no. 5 carried a nucleolar organizer region (NOR) composed of a cluster of rDNA genes as confirmed by both the classical banding approaches and FISH with 18S rDNA probe. In two acanthocephalan sibling species Pomphorhynchus laevis and P. tereticollis (Acanthocephala, both 2n = 6 + XX in female and X0 in male), two NORs were found, located each in different sites of the first two autosomal pairs. Furthermore, we hybridized the "vertebrate" telomeric probe, (TTAGGG)n, with chromosomes of above-mentioned helminths and cestodes Caryophyllaeus laticeps(2n = 20), Caryophyllaeides fennica (2n = 20), and Nippotaenia mogurndae (2n = 28). The probe hybridized with chromosome ends in all Platyhelminthes (Monogenea, Cestoda) but not in Acanthocephala. No hybridization signals in P. laevis and P. tereticollis were also obtained with the "arthropod" (TTAGG)n or "nematode"(TTAGGC)n telomeric probes. Our results suggest that whereas the telomeres in Platyhelminthes are composed of the TTAGGG repeates, this ancestral motif of telomeres in Bilateria was lost in Acanthocephala and replaced with unknown telomeric sequences.
ER  -

BOMBAROVÁ, Marta, Magda VÍTKOVÁ, František MAREC, Martina PEČÍNKOVÁ, Marta ŠPAKULOVÁ a Ivica HROMADOVÁ. Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala. In \textit{11th International Congress of Parasitology, CD-ROM}. Glasgow, Scotland: ICOPA XI Congress Secretariat, 2006, s.~CD-ROM, 1 s.

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