Invasion of ED-1+ macrophages and changes of molecular
expression in the contralateral homologous DRG following
unilateral sciatic nerve injury

D 2006

Invasion of ED-1+ macrophages and changes of molecular expression in the contralateral homologous DRG following unilateral sciatic nerve injury

DUBOVÝ, Petr; Lucie TUČKOVÁ; Kateřina PEJCHALOVÁ; Ilona KLUSÁKOVÁ; Ivana SVÍŽENSKÁ et al.

Základní údaje

Originální název

Invasion of ED-1+ macrophages and changes of molecular expression in the contralateral homologous DRG following unilateral sciatic nerve injury

Název česky

Invaze ED-1+ makrofágů a změny v expresi molekul v kontralaterálních spinálních gangliích po unilaterálním poškození nervu

Autoři

DUBOVÝ, Petr; Lucie TUČKOVÁ; Kateřina PEJCHALOVÁ; Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ

Vydání

Praha, Morphology 2006, od s. 52-52, 1 s. 2006

Nakladatel

UK Praha

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

dorsal root ganglia - cellular changes - molecular changes

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 2. 2010 17:39, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

Due to the highly symmetrical organization of the peripheral nervous system, changes on the injured side were often compared to the uninjured contralateral side as control. However, within the last years, a growing number of reports of bilateral effects after unilateral peripheral nerve injury has been published. Unilateral ligature of the sciatic nerve was performed aseptically under deep anesthesia in 12 rats. Further 6 rats were sham-operated, and 4 rats without operation were used as naive control. All animals were left to survive for 1, 2, and 4 weeks. The L4-L5 dorsal root ganglia (DRG) were removed from ipsi- and contralateral side, and cryostat sections were incubated for immunofluorescence detection of ATF3, pCREB, TNFa, TNFR1, IL-1b, IL-6, SDF-1, and CXCR4. Macrophages invading DRG were detected by ED-1 immunostaining. In comparison to the DRG from naive rats, the qualitative and quantitative changes of immunofluorescence staining for the above-mentioned molecules excluding ATF3 were observed not only in the ipsi-, but also in homologous contralateral DRG during 1 to 4 weeks from operation. Invasion of ED-1+ macrophages was statistically increased in both ipsi- and contralateral DRG only after 4 weeks. The results indicate that contralateral DRG not affected directly by nerve injury display cellular and molecular changes that may contribute to a misbalance of signals from peripheral structures to CNS.

Česky

Invaze makrofágů probíhá nejen do ipsi-, ale také do kontralaterálních spinálních ganglií po unilaterálním poškození periferního nervu.

Návaznosti

MSM0021622404, záměr

Název: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Citovat

DUBOVÝ, Petr; Lucie TUČKOVÁ; Kateřina PEJCHALOVÁ; Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ. Invasion of ED-1+ macrophages and changes of molecular expression in the contralateral homologous DRG following unilateral sciatic nerve injury. In Morphology 2006. Praha: UK Praha, 2006, s. 52-52.

@inproceedings{707059,
   author = {Dubový, Petr and Tučková, Lucie and Pejchalová, Kateřina and Klusáková, Ilona and Svíženská, Ivana},
   address = {Praha},
   booktitle = {Morphology 2006},
   keywords = {dorsal root ganglia - cellular changes - molecular changes},
   language = {eng},
   location = {Praha},
   pages = {52-52},
   publisher = {UK Praha},
   title = {Invasion of ED-1+ macrophages and changes of molecular expression in the contralateral homologous DRG following unilateral sciatic nerve injury},
   year = {2006}
}

TY  - CONF
ID  - 707059
AU  - Dubový, Petr - Tučková, Lucie - Pejchalová, Kateřina - Klusáková, Ilona - Svíženská, Ivana
PY  - 2006
TI  - Invasion of ED-1+ macrophages and changes of molecular expression in the contralateral homologous DRG following unilateral sciatic nerve injury
PB  - UK Praha
CY  - Praha
KW  - dorsal root ganglia - cellular changes - molecular changes
N2  - Due to the highly symmetrical organization of the peripheral nervous system, changes on the injured side were often compared to the uninjured contralateral side as control. However, within the last years, a growing number of reports of bilateral effects after unilateral peripheral nerve injury has been published. Unilateral ligature of the sciatic nerve was performed aseptically under deep anesthesia in 12 rats. Further 6 rats were sham-operated, and 4 rats without operation were used as naive control. All animals were left to survive for 1, 2, and 4 weeks. The L4-L5 dorsal root ganglia (DRG) were removed from ipsi- and contralateral side, and cryostat sections were incubated for immunofluorescence detection of ATF3, pCREB, TNFa, TNFR1, IL-1b, IL-6, SDF-1, and CXCR4. Macrophages invading DRG were detected by ED-1 immunostaining. In comparison to the DRG from naive rats, the qualitative and quantitative changes of immunofluorescence staining for the above-mentioned molecules excluding ATF3 were observed not only in the ipsi-, but also in homologous contralateral DRG during 1 to 4 weeks from operation. Invasion of ED-1+ macrophages was statistically increased in both ipsi- and contralateral DRG only after 4 weeks. The results indicate that contralateral DRG not affected directly by nerve injury display cellular and molecular changes that may contribute to a misbalance of signals from peripheral structures to CNS.
ER  -

DUBOVÝ, Petr; Lucie TUČKOVÁ; Kateřina PEJCHALOVÁ; Ilona KLUSÁKOVÁ a Ivana SVÍŽENSKÁ. Invasion of ED-1+ macrophages and changes of molecular expression in the contralateral homologous DRG following unilateral sciatic nerve injury. In \textit{Morphology 2006}. Praha: UK Praha, 2006, s.~52-52.

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