| HEJÁTKO, Jan, I. BLILOU, P. B BREWER, Jiří FRIML, B. SCHERES a Eva BENKOVA. In situ hybridisation technique for mRNA detection in whole mount Arabidopsis samples. Nature Protocols. U.S.A.: Nature Publishing Group, 2006, roč. 1, č. 3, s. 1462 – 1467, 8 s. ISSN 1745-2473. |
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@article{707314,
author = {Hejátko, Jan and Blilou, I. and Brewer, P. B and Friml, Jiří and Scheres, B. and Benkova, Eva},
article_location = {U.S.A.},
article_number = {3},
keywords = {in situ mRNA localization; whole mount; Arabidopsis},
language = {eng},
issn = {1745-2473},
journal = {Nature Protocols},
title = {In situ hybridisation technique for mRNA detection in whole mount Arabidopsis samples.},
volume = {1},
year = {2006}
}
TY - JOUR
ID - 707314
AU - Hejátko, Jan - Blilou, I. - Brewer, P. B - Friml, Jiří - Scheres, B. - Benkova, Eva
PY - 2006
TI - In situ hybridisation technique for mRNA detection in whole mount Arabidopsis samples.
JF - Nature Protocols
VL - 1
IS - 3
SP - 1462 – 1467
EP - 1462 – 1467
PB - Nature Publishing Group
SN - 17452473
KW - in situ mRNA localization
KW - whole mount
KW - Arabidopsis
N2 - High throughput microarray transcription analyses provide us with the expression profiles for large amounts of plant genes. However, their tissue and cellular resolution is limited. Thus, for detailed functional analysis, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here, we present an in situ mRNA hybridisation method that is routinely used for the analysis of plant gene expression patterns. The protocol is optimised for whole mount mRNA localisations in Arabidopsis seedling tissues including embryos, roots, hypocotyls and young primary leaves. It can also be used for comparable tissues in other species. Part of the protocol can also be automated and performed by a liquid handling robot. Here we present a detailed protocol, recommended controls and troubleshooting, along with examples of several applications. The total time to carry out the entire procedure is ~7 d, depending on the tissue used.
ER -
HEJÁTKO, Jan, I. BLILOU, P. B BREWER, Jiří FRIML, B. SCHERES a Eva BENKOVA. In situ hybridisation technique for mRNA detection in whole mount Arabidopsis samples. \textit{Nature Protocols}. U.S.A.: Nature Publishing Group, 2006, roč.~1, č.~3, s.~1462 – 1467, 8 s. ISSN~1745-2473.
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